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MiPEPs are microproteins encoded by primary transcripts of microRNAs (pri-miRNAs). Initially identified in plants, we recently characterized a miPEP in Drosophila melanogaster, named miPEP8, which is involved in the regulation of wing size. However, mechanisms …
Read PDFHidradenitis suppurativa (HS) is an autoinflammatory keratinisation disease affecting the pilosebaceous unit and hallmarked by a complex and multifactorial pathogenesis. Although genomic and transcriptomic investigations have substantially advanced our understanding of key mechanisms underlying HS …
Read PDFAge-related macular degeneration (AMD), a chronic inflammatory disease, is a major cause of irreversible blindness worldwide. It involves the degeneration of the retinal pigment epithelium (RPE) and the accumulation of deposits between the RPE and …
Read PDFHaploinsufficiency of TBK1 causes familial ALS and frontotemporal dementia (FTD), yet the mechanisms by which TBK1 loss leads to neurodegeneration remain unclear. Using deep proteomics and phospho-proteomics, we demonstrate that TBK1 regulates select macroautophagy/autophagy factors, …
Read PDFProstate cancer (PCa) is common world-wide. Current diagnostics based on testing for circulating levels of prostate specific antigen (PSA) is unspecific, and novel prognostic markers are needed for personalized therapeutic strategies. Pro-neuropeptide Y (pro-NPY) has …
Read PDF<h4>Introduction</h4>Visual dysfunction and retinal structural changes can occur early in Alzheimer's disease (AD), but the molecular basis of these early alterations remains unclear.<h4>Methods</h4>We performed quantitative proteomic profiling of the retina and brain from 4-week-old triple-transgenic …
Read PDFThe role of plasma and serum proteomics in characterizing human disease, identifying biomarkers, and advancing diagnostic technologies is rapidly increasing. However, there is an ongoing need to improve proteomic workflows in terms of accuracy, reproducibility, …
Read PDFCirculating blood proteomics enables minimally invasive biomarker discovery. Nanoparticle-based circulating plasma proteomics studies have reported varying number of proteins (ca 2000-7000), but it remains unclear whether a higher protein number is more informative. Here, we …
Read PDF<b>Background</b>: Zangqing '1127', a hull-less barley type recognized for its high β-glucan content, holds significant agricultural and nutritional potential. Nonetheless, the molecular mechanisms underlying the degradation of β-glucan during barley germination have yet to be …
Read PDFIntestinal mucin glycan-degrading bacteria are important for mucus turnover, stimulating mucus production, and producing beneficial metabolites. The mucin-degrading bacteria require various enzymes to break down mucin O-glycans. In this study, mucin glycan-degrading bacteria Akkermansia muciniphila, …
Read PDF<h4>Background/aims</h4>Hepatocellular carcinoma (HCC) is one of the leading causes of cancer-related mortality worldwide and is driven by metabolic reprogramming that supports tumor growth and progression. A common missense genetic variant (rs2642438, p.A165T) in mitochondrial amidoxime …
Read PDFBottom-up proteomics is a powerful technique for comprehensive analysis of proteins by proteolytic cleavage followed by LC-MS/MS to identify the resulting peptides. Trypsin is the gold-standard protease for bottom-up proteomics, though its cleavage specificity limits …
Read PDF<h4>Background</h4>Aflatoxin B<sub>1</sub> (AFB<sub>1</sub>), a potent mycotoxin, commonly contaminates feeds like maize and soybean, jeopardizing animal reproduction. Although AFB<sub>1</sub> exposure is known to cause oxidative stress, immune activation, and cell death in oocytes and early embryos …
Read PDFCapillary zone electrophoresis (CZE)-mass spectrometry (MS) has attracted tremendous attention in top-down proteomics (TDP). However, its reproducibility and long-term repeatability for TDP remain concerns, most likely due to capillary coating. Here, we present an improved …
Read PDFBACKGROUND: The current phenotyping of chronic rhinosinusitis with nasal polyps (CRSwNP) into eosinophilic (eCRSwNP) and non-eosinophilic (non-eCRSwNP) subtypes is increasingly insufficient to address complex clinical challenges, especially as some non-eCRSwNP patients have poor prognoses. Identifying …
Read PDFMany diseases, including diabetes and hypertension, can lead to kidney damage, often resulting in long-term health complications and organ failure. Both animal and human studies have documented a causal relationship between high dietary sodium intake …
Read PDFLon protease is a global regulator in bacteria affecting biofilm formation, antibiotic resistance, toxin-antitoxin complexes, and proteolysis of damaged proteins. In <i>Klebsiella pneumoniae</i>, Lon has roles in iron response, protein transport, and membrane assembly. Here, …
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