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<h4>Objectives</h4>CRISPR-Cas9 nucleases are widely used to introduce targeted DNA double-strand breaks (DSBs) for genome engineering, but the long-term impact of these lesions on local epigenetic information remains poorly characterized. In a companion research article, we …
Read PDFIntegrated Mangrove Aquaculture (IMA) and Sustainable Aquaculture in Mangrove Ecosystem Fisheries (SAIME) are key activities undertaken across coastal regions globally to meet growing demand for brackish-water aquaculture products through sustainable practices. An in-depth biomonitoring study …
Read PDFDNA replication stress is a hallmark of cancer that is exploited by chemotherapies. Current assays for replication stress have low throughput and poor resolution whilst being unable to map the movement of replication forks genome-wide. …
Read PDFWe report fatal tularemia in stranded beluga whales in Cook Inlet, Alaska, USA. Francisella tularensis was detected by nanopore metagenomics, confirmed by quantitative PCR and immunohistochemistry, and characterized as F. tularensis subspecies holarctica by multilocus …
Read PDF<h4>Objectives</h4>Thermophilic bacteria are valuable biotechnological resources. A thermophilic bacterium isolated from the Thai hot spring sediments was subjected to complete genome sequencing. Genome data will facilitate bacterial identification and comparative genomics within the Hydrogenophilus genus …
Read PDFUnderstanding the host transcriptional and epitranscriptomic response to <i>Mycobacterium tuberculosis</i> (Mtb) infection is vital for decoding mechanisms of immune evasion and persistence. Here, we employed Oxford Nanopore Technologies (ONT)-based direct RNA sequencing (DRS) on human …
Read PDF<i>Pseudomonas fulva</i> MTT5, isolated from maize leaf surfaces, exhibits broad-spectrum antimicrobial activity. We present the complete genome sequence of <i>P. fulva</i> MTT5. The 4.9 Mb genome contains 4,488 predicted genes and a GC content of …
Read PDF<i>Burkholderia pseudomallei</i> is a soil-dwelling bacterium causing melioidosis, a severe and often fatal disease (10-40% lethality). Here, we report 20 complete high-quality, closed genomes (7.0-7.5 Mbp, ~68.1% GC) from Vietnamese soil isolates, assembled from Oxford …
Read PDF<i>Bacillus</i> contamination in plant-based food products is a significant concern due to heat-resistant spores that can survive heating and proliferate during storage or handling, possibly leading to foodborne illnesses. Given the potential of high-throughput DNA …
Read PDFTigridiopalma magnifica, a member of the Melastomataceae family, is a monotypic genus perennial plant species endemic to South China. Due to its rarity and restricted distribution, the species has been designated as a national second-level …
Read PDF<h4>Background</h4>The characterisation of circulating nucleic acid biomarkers in liquid biopsy based diagnostics holds great potential to transform the landscape of early cancer detection and screening. These tests are increasingly incorporating information beyond the primary sequence, …
Read PDFPortulacaria afra (P. afra), or Spekboom as it is locally known, is a versatile evergreen shrubby succulent plant in the family Didiereaceae and endemic to the south-eastern parts of southern Africa. P. afra is not …
Read PDFGiardia duodenalis, a protozoan parasite of important zoonotic concern, is estimated to cause approximately 280 million human infections annually worldwide. Currently, genome sequencing of G. duodenalis mainly relies on in vitro axenic clones; however, for …
Read PDFHairy/Enhancer of Split (HES) proteins are critical for animal development and for the regulation of human diseases. The <i>Caenorhabditis elegans</i> genome encodes six HES orthologs, including HLH-29 . We used Nanopore sequencing on the MinION …
Read PDFSeveral arboviruses including Dengue, Chikungunya, Zika, West Nile and Japanese encephalitis viruses are emerging and re-emerging in many parts of the world over last two decades. Thus, environmental surveillance of the mosquito borne viruses employing …
Read PDF<h4>Introduction</h4>In immunohematology case studies the knowledge about variants of the blood group gene of interest can facilitate antibody diagnosis. Known gene variants can be rapidly genotyped by specific methods, but the identification of unknown variants …
Read PDFChemical risk assessment can benefit from integrating informative biomarkers in human biomonitoring (HBM). Beyond exposure biomarkers, effect biomarkers inform on biological reactions in the body, potentially leading to adverse effects, while susceptibility biomarkers address inter-individual …
Read PDF<i>Burkholderia gladioli</i> is a Gram-negative bacterium with multi-host adaptability as both a plant pathogen and an opportunistic human pathogen. It is also known as a producer of diverse bioactive metabolites. This dataset presents the first …
Read PDF<h4>Background</h4>Genetic defects in von Willebrand factor (VWF) can lead to von Willebrand disease (VWD). Identifying causative or modifier variants of <i>VWF</i> is crucial for the diagnosis, classification, and clinical management of VWF disorders. However, owing …
Read PDFWhole-genome sequencing provides the highest resolution for characterizing pathogen evolution, epidemiology, and diagnostics. Genome assemblies contain information on the identity and potential phenotypes of a pathogen. Likewise, variant calling can inform on transmission patterns and …
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