Location and organization of a complex, integrated transgenic array in a <i>Caenorhabditis elegans</i> strain carrying <i>hlh-29p::gfp</i>

Hairy/Enhancer of Split (HES) proteins are critical for animal development and for the regulation of human diseases. The <i>Caenorhabditis elegans</i> genome encodes six HES orthologs, including HLH-29 . We used Nanopore sequencing on the MinION platform to define the location and organization of an integrated transgenic array expressing green fluorescent protein driven by the <i>hlh-29</i> promoter. The array, <i>ardIS501</i> , is at least 188.5 kb long, is inserted into Chromosome III of Bristol N2 , and contains at least 26 copies of <i>hlh-29 p::gfp</i> and 11 copies of <i>rol-6 ( su1006 )</i> . The coding sequences <i>Y46E12A.2 .1</i> and <i>Y46E12A.5 .1</i> are deleted in this strain, with no observable phenotypes.