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<h4>Background</h4>Oxford Nanopore sequencing is a long-read sequencing technology that does not rely on a polymerase to generate sequence data. Sequencing library preparation methods used in Oxford Nanopore sequencing rely on the addition of a motor …
Read PDFWe report the complete genome sequence of plant growth-promoting rhizobacteria strain WSM3457. The genome consists of a single 7.1 Mbp chromosome, with phylogenetic and average nucleotide identity comparisons indicating the strain is <i>Pseudomonas protegens</i> WSM3457.
Read PDFWe report the draft genome sequence of <i>Staphylococcus saprophyticus</i> strain of Nasal Sample 2 (NS2) isolated from a milkman handling cows with subclinical mastitis in Kiruhura District, Uganda. The assembled genome comprises 2,773,281 bp with …
Read PDFAvian haemosporidian parasites are vector-borne apicomplexans that infect bird species globally and pose considerable challenges in detection due to frequent co-infections and morphological convergence. In the present study, we first used Oxford Nanopore Technologies (ONT) …
Read PDFWe report the complete genome sequence of <i>Cysteiniphilum</i> strain GTC20157 isolated from coastal seawater in Osaka, Japan. The genome comprised a circular chromosome and a circular plasmid measuring 2,508,673 and 149,142 bp, respectively.
Read PDFWe report whole-genome sequences of 20 <i>Bacillus cereus sensu lato</i> strains from plant-based fermentations, foodborne outbreaks, environmental sources, and dairy plants. Genomic DNA was extracted and sequenced using Oxford Nanopore Technology. These complete assemblies provide …
Read PDFLumpy Skin Disease (LSD) is a transboundary viral disease of wild and domesticated ruminants, and notifiable to the World Organisation for Animal Health (WOAH). LSD has spread rapidly in the cattle population across the African, …
Read PDFWe report the draft genome sequence of <i>Babesia gibsoni</i> detected in a domestic dog in New Zealand. A high-quality near-complete genome, comprised of an apicoplast, mitochondrion, and four chromosomes, was produced through long-read metagenomic sequencing.
Read PDF<b>Background</b> <i>. Klebsiella pneumoniae</i> (<i>Kpn</i>) is an important cause of healthcare-associated infections (HAIs). In low- and middle-income countries, HAI due to <i>Kpn</i> disproportionately affects neonates. In this study, we investigated the genomic changes that occurred …
Read PDF<h4>Background</h4>Cervical cancer due to high-risk human papillomavirus (HR-HPV) is the leading gynecological cancer in Sub-Saharan Africa and in the Democratic Republic of the Congo (DRC), where approximately one-quarter of people carries the sickle cell allele. …
Read PDFMany diseases are caused by pathogenic expansion of microsatellite repeats. Longread sequencing allows evaluation of the content of such expanded repeats. Friedreich ataxia patients are typically homozygous for an expanded GAA repeat in intron 1 …
Read PDFThe complete genome of <i>Bifidobacterium animalis</i> subsp. <i>lactis</i> BI040-a human stool isolate, was sequenced using Illumina and Oxford Nanopore technologies. The BI040 genome is composed of a circular 1,944,141-bp chromosome which carries genes potentially involved …
Read PDFSequencing of the <i>Lacticaseibacillus rhamnosus</i> NS2301G1 strain isolated from kimchi revealed a 2,968,519 bp circular chromosome with a 46.74% GC content; in addition, genes linked to probiotic properties, stress tolerance, and carbohydrate metabolism were identified, …
Read PDF<i>Ekhidna algicida</i> To15<sup>T</sup> was isolated from the Tropical Pacific Ocean, based on its algicidal effect on the pelagic diatom <i>Thalassiosira oceanica</i>. We report the complete genome sequence, comprising one chromosome for this newly discovered species …
Read PDFMicrobiome research has shown significant potential in enhancing poultry health and productivity. Despite the increasing volume of data linking the microbiota with various host traits, challenges persist regarding the consistency and reproducibility of findings. A …
Read PDF<h4>Background</h4>The aim of this study was to compare microbial diversity and compositional changes under digestive stress in yogurts produced using different culture strategies in a dynamic in vitro gastrointestinal system.<h4>Methods</h4>Yogurts produced with probiotic starter cultures, …
Read PDF<h4>Background</h4>Oxford Nanopore Technologies (ONT) sequencing enables direct, long-read sequencing of DNA and RNA, preserving nucleotide modifications. During basecalling, deep neural networks translate raw nanopore signals into nucleotide sequences, internally segmenting the signal to align it …
Read PDFPetroleum pollution poses a critical environmental concern. Bioremediation has gained prominence as an eco-friendly approach for mitigating hydrocarbon pollution. This study reports the isolation and comprehensive characterization of a novel petroleum-degrading bacterium, Rhodococcus indonesiensis SARSHI1. …
Read PDF<h4>Background</h4>Sorghum bicolor and Sorghum halepense can readily hybridize, creating difficulty in identification. No genetic tools exist to accurately distinguish S. bicolor, S. halepense, and their hybrids. Detecting hybridization is essential to monitor crop-to-weed introgression. This …
Read PDFThe genus <i>Eubacterium</i> comprises gram-positive anaerobic bacteria. It has long been recognized as a component of human oral and gut microbiota. Here, we report the complete genome sequence of a novel strain, <i>Eubacterium brachy</i> GTC …
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