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While chemical fertilizers (CF) ensure rapid crop growth, relying exclusively on them can disrupt natural nutrient cycling and lead to environmental concerns such as nutrient imbalance. In contrast, organic amendments offer a sustainable alternative by …
Read PDFNext generation long-read sequencing is a powerful approach to generate de novo genome assemblies, however it requires high-quality and high molecular weight (HMW) DNA to reach near-chromosome level assemblies. Some plants are reported as recalcitrant …
Read PDFWe report the complete genome sequence of a clinical isolate of <i>Desulfovibrio legallii</i> obtained from a human patient in Japan. The genome consists of a 2,750,737-bp circular chromosome.
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Read PDFHere, we report the genomic sequence of <i>Vibrio pectenicida</i> strain FHCF-3, isolated from the coelomic fluid of a sunflower sea star (<i>Pycnopodia helianthoides</i>) showing signs of wasting disease. The draft genome is 4,368,354 bp with …
Read PDFThe sulfide-oxidizing bacterium <i>Alkalilimnicola ehrlichii</i> strain SGAR-12 was isolated from a pilot-scale haloalkaline biodesulfurization (BD) installation, and its genome was sequenced. Additionally, the genome of the type strain of the species, strain MLHE<sup>T</sup>, was sequenced. …
Read PDF<h4>Background</h4>Intracranial infections are severe, life-threatening conditions often caused by diverse pathogens. Targeted nanopore sequencing (TNPseq) offers rapid and accurate pathogen identification, addressing the limitations of traditional diagnostic methods. Besides the pathogen types, factors like inflammation …
Read PDF<b>Background/Objectives:</b> Massively parallel sequencing technologies have advanced chronic lymphocytic leukemia (CLL) diagnostics and precision oncology. Illumina platforms, while offering robust performance, require substantial infrastructure investment and a large number of samples for cost-efficiency. Conversely, third-generation …
Read PDFThe Oxford Nanopore platform and nanopore sequencing are gaining increasing popularity in modern metagenomic research. However, there is a limited set of dedicated tools for analyzing this type of data. The tools used for nanopore …
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Read PDF<h4>Background</h4>Recent evidence suggests that human semen harbors a complex microbiome that may influence semen quality and male fertility. However, comprehensive characterization of bacterial and fungal communities in semen remains limited, particularly using long-read sequencing technologies.<h4>Methods</h4>In …
Read PDFAlternative splicing is a key regulator of immune regulation by enabling rapid and context-specific responses. However, the role of splicing regulators such as CDC-like kinase 1 (CLK1) in monocyte biology remains poorly defined. Here, we …
Read PDFA Gram-stain-negative, strictly aerobic, non-motile, non-flagellated and rod-shaped bacterium, strain MW10<sup>T</sup>, was isolated from freshwater in South Korea. The strain exhibited catalase-negative and oxidase-positive activities and grew at 15-35 °C (optimum, 25 °C), pH 6.0-9.0 …
Read PDFFree-roaming cats may contribute to zoonotic risk via parasites and other eukaryotic taxa, yet surveillance in protected island settings is limited and conventional coprology can miss low-intensity or degraded signals. We conducted a cross-sectional 18S …
Read PDFThe identification of structural variant (SV) breakpoints plays a crucial role in understanding the genetic variants, mutagenic mechanisms, and functional consequences that drive various genetic diseases. While next-generation sequencing (NGS) has become a cornerstone in …
Read PDFBangladesh's aquaculture is currently faced with <i>Vibrio</i> contamination, which poses a threat to public health, exports, and product quality. This research provides a detailed molecular characterization of <i>Vibrio</i> sp. found in shrimp through the use …
Read PDFArtisanal cheese quality relies on a complex microbiota. The generalized use of commercial starter cultures has been associated with reduced microbial diversity, fueling interest in using indigenous lactic acid bacteria (LAB) as adjunct cultures. This …
Read PDFAnaerobic degradation of aromatic hydrocarbons such as toluene plays a critical role in the natural and engineered attenuation of contaminated environments. Here, we developed and characterized a microbial consortium enriched under strictly anoxic conditions, capable …
Read PDFThe combination of ultra-long (UL) Oxford Nanopore Technologies (ONT) sequencing reads with long, accurate Pacific Bioscience (PacBio) High Fidelity (HiFi) reads has enabled the completion of a human genome and spurred similar efforts to complete …
Read PDFBACKGROUND: Bats are recognised as reservoirs for a wide range of microorganisms, including viruses, bacteria, fungi, and parasites, some of which are of zoonotic concern. The common vampire bat (Desmodus rotundus) is particularly important due …
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