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A cell's transcriptome is regulated through the integration of external and internal signals that activate intracellular signal pathways, epigenetic modifications and post-translational changes. Post-transcriptional regulation through RNA methylation has emerged as an important mechanism in …
Read PDFOur study investigates the effectiveness of Oxford Nanopore Technologies for accurate outbreak tracing by resequencing 33 isolates of a 3-year-long <i>Klebsiella pneumoniae</i> outbreak with Illumina short-read sequencing data as the point of reference. We detect …
Read PDFThe genome sequences of two <i>Thioalkalivibrio sulfidiphilus</i> strains, SGAR-7 and SGAR-13, isolated from a pilot-scale haloalkaline biodesulfurization (BD) installation, are reported. The genomes comprise Flavocytochrome c sulfide dehydrogenase and SoxAX cytochromes, which are essential for …
Read PDF<h4>Background</h4>Long-read technologies from Pacific Biosciences (PacBio) and Oxford Nanopore Technologies (ONT) have transformed genomics research by providing diverse data types like HiFi, Duplex, and ultra-long ONT. Despite recent strides in achieving haplotype-phased gapless genome assemblies …
Read PDFWe report the complete genome of <i>Enterococcus faecium</i> PM1, isolated from the gizzard of a diseased broiler breeder in a commercial farm experiencing recurrent disease outbreaks. The genome includes a 2,520,275 bp circular chromosome and …
Read PDF<i>Wolbachia</i> are widespread insect endosymbionts known for manipulating host reproduction, aiding vector-borne disease control, and influencing host evolution. Here, we provide the complete genome sequence of the <i>Wolbachia</i> strain <i>w</i>Mel derived from the <i>Drosophila melanogaster</i> …
Read PDFAs nanopore sequencing has been widely adopted, data accumulation has surged, resulting in over 700,000 public datasets. While these data hold immense potential for advancing genomic research, their utility is compromised by the absence of …
Read PDFLocal protein synthesis in neurons is vital for synaptic maintenance and plasticity, yet the regulatory mechanisms, particularly cytoplasmic polyadenylation, are not fully understood. This study used nanopore sequencing to examine transcriptomic responses and 3'-end dynamics …
Read PDFA unique feature of Oxford Nanopore Technologies sequencers, adaptive sampling, allows precise DNA molecule selection from sequencing libraries. Here, we present enhancements to our tool, readfish, enabling all features for the industrial scale PromethION sequencer, …
Read PDF<h4>Background</h4>Culturing of fungi is labor-intensive and reveals limited diversity, while high-throughput sequencing of barcodes (i.e., metabarcoding) enables a simultaneous detection of fungi from multiple environmental samples. Metabarcoding using short-read sequencers, such as Illumina platforms, provides …
Read PDFMore than 60 human disorders are caused by unstable expansion of short tandem repeat (STR) tracts. These can exhibit cell-type-specific mosaicism in several repeat expansion disorders and remain difficult to characterize due to technical challenges …
Read PDF<h4>Background</h4>Mycena is a widespread genus of mushroom capable of decomposing various dead plant substrates. Gastrodia elata is a fully mycoheterotrophic orchid whose seed germination depends on specific Mycena strains. However, only a few Mycena species …
Read PDFSarcandra glabra (Thunb.) Nakai, a member of the family Chloranthaceae, has a rich history of use in traditional Chinese medicine. To date, >400 compounds have been isolated and characterized from this plant. Studying the genome …
Read PDFBACKGROUND: After the first case of COVID-19 was reported in Côte d’Ivoire in March 2020, the virus spread significantly, with several epidemic waves. During a carriage study conducted from November 2020 to April 2021 to …
Read PDFBACKGROUND: Since 2019, Cameroon has reported a high number of seasonal influenza cases caused by the A(H1N1)pdm09 subtype, which remained the predominant global strain as of 2024. METHODS: To characterize the evolutionary dynamics of circulating …
Read PDFPorcine biomedical models have emerged as valuable tools in biomedical research due to their physiological, anatomical, metabolic, immunological, and genetic similarities to humans. As a result, they offer greater relevance for translational studies than rodent …
Read PDFCarbapenemase-producing Enterobacterales (CPE) are a major public health concern. Within the <i>Enterobacter cloacae</i> complex (ECC), the <i>bla</i><sub>VIM-1</sub> carbapenemase gene is frequently plasmid-borne, enabling inter-clonal and inter-species spread that complicates the detection and control of carbapenemase …
Read PDFMonkeypox (Mpox), a zoonotic disease caused by the mpox virus, has been endemic mainly in West and Central Africa for a long time, with only a few cases in other parts of the world. However, …
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