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Full text 2025

Complete genome assembly and functional characterization of <i>Brucella melitensis</i> strain IMHB1 from a clinical isolate in Inner Mongolia, China

Zheng X, Liang C, Shao L, et al.

Brucellosis is a globally prevalent zoonotic disease caused by <i>Brucella</i> species, posing a significant threat to both public health and the livestock industry. Despite ongoing research efforts, the mechanisms underlying <i>Brucella</i> pathogenesis remain poorly understood, …

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Nanopore Sequencing
Full text 2025

GoldPolish-target: targeted long-read genome assembly polishing

Zhang E, Coombe L, Wong J, et al.

<h4>Background</h4>Advanced long-read sequencing technologies, such as those from Oxford Nanopore Technologies and Pacific Biosciences, are finding a wide use in de novo genome sequencing projects. However, long reads typically have higher error rates relative to …

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Nanopore Sequencing
Full text 2025

Comprehensive genomic insights into &lt;i&gt;Mixta calida&lt;/i&gt; isolated from the faecal sample of a tuberculosis patient in India

Pant A, Srinivasan J, Venkadesan S, et al.

<i>Mixta calida</i>, formerly known as <i>Pantoea calida</i>, is a motile Gram-negative, facultatively anaerobic bacterium with coccoid rod morphology. Although previously considered non-pathogenic, emerging case studies indicate its potential role in causing serious infections, including bacteraemia, …

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Nanopore Sequencing
Full text 2026

Rawsamble: overlapping raw nanopore signals using a hash-based seeding mechanism

Firtina C, Mordig M, Mustafa H, et al.

<h4>Motivation</h4>Raw nanopore signal analysis is a common approach in genomics to provide fast and resource-efficient analysis without translating the signals to bases (i.e. without basecalling). However, existing solutions cannot interpret raw signals directly if a …

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Basecalling Genome Assembly
Full text 2025

Nuclear and organelle genome assemblies of 5 Cucumis melo L. accessions, Ananas, Canton, PI 414723, Vedrantais, and Zhimali, belonging to diverse botanical groups

Belinchon-Moreno J, Berard A, Canaguier A, et al.

The construction of accurate whole genome sequences is pivotal for characterizing the genetic diversity of plant species, identifying genes controlling important traits, or understanding their evolutionary dynamics. Here, we generated the nuclear, mitochondrial, and chloroplast …

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Nanopore Sequencing
Full text 2025

Haplotype-resolved genome assembly of the tetraploid potato cultivar Désirée

Godec T, Beier S, Rodriguez-Granados NY, et al.

Cultivar Désirée is an important model for potato functional genomics studies to assist breeding strategies. Here, we present a haplotype-resolved genome assembly of Désirée, achieved by assembling PacBio HiFi reads and Hi-C scaffolding, resulting in …

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Nanopore Sequencing
Full text 2025

Colora: a Snakemake workflow for complete chromosome-scale de novo genome assembly

Obinu L, Booth T, De Weerd H, et al.

<h4>Motivation</h4>De novo assembly creates reference genomes that underpin many modern biodiversity and conservation studies. Large numbers of new genomes are being assembled by labs around the world. To avoid duplication of efforts and variable data …

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Genome Assembly Nanopore Sequencing Workflows & Pipelines