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<b>Background/Objectives</b>: BrdU (5'-bromo-2'-deoxyuridine), a synthetic thymidine (T) analog, is widely used to study cell proliferation and DNA synthesis. To precisely identify where and when DNA replication starts and terminates, it is essential to determine the …
Read PDF<i>Aedes albopictus</i> has recently established self-sustaining populations in Hungary, but its microbiota-which may influence vector competence-remains poorly understood. We used Oxford Nanopore long-read sequencing for full-length 16S rRNA gene profiling of adult <i>Ae. albopictus</i> from …
Read PDF<h4>Background</h4>Central nervous system (CNS) infections pose a significant public health challenge in resource-limited settings. Traditional culture-based and targeted molecular diagnostic methods have limitations in sensitivity and speed. This study retrospectively analyzed the data and cerebrospinal …
Read PDFIn March of 2025 the UK's first Marine Citizen Science Week recruited Citizen Scientists from around the country to participate in a series of activities exploring the ecology and biodiversity of rocky shorelines at sites …
Read PDFWe report the complete genome of <i>Burkholderia pseudomallei</i> strain 490f, isolated from a ceftazidime treatment failure case in Northeast Thailand. Oxford Nanopore and Illumina sequencing techniques revealed two circular chromosomes. A 150 kb region on …
Read PDFHypsibarbus vernayi (2n = 50), a medium-sized barb distributed in the Mekong River basin, is widely consumed in its native range and holds significant potential for commercial aquaculture development. To date, there is no high-quality …
Read PDFDNA methylation is a key epigenetic modification involved in various biological processes. However, its role in <i>Nosema ceranae</i> remains poorly understood. In this study, we employed Oxford Nanopore Technology sequencing to identify whole-genome DNA methylation …
Read PDF<b>Background/Objective</b>: Timely identification of bloodstream pathogens is critical for sepsis management; however, PCR inhibitors such as sodium polyanetholesulfonate (SPS) in blood culture broth compromise nucleic acid recovery and long read sequencing. We assessed whether coupling …
Read PDFRecent studies have revealed that the long-recognized link between the historically defined <i>Fusobacterium nucleatum</i> group and colorectal cancer is largely driven by <i>Fusobacterium animalis</i>. This species, along with two others (<i>Fusobacterium polymorphum</i> and <i>Fusobacterium vincentii</i>), …
Read PDF<h4>Background</h4>Long-read sequencing has revolutionised metagenomics through improved metagenome assembly, taxonomic classification and functional characterisation. Automation can enhance the throughput, reproducibility, and accuracy of library preparation. However, the validation of automated library preparation protocols remains undetermined …
Read PDFThe Bonin flying fox (Pteropus pselaphon) is an endemic species of the Ogasawara Islands, Japan, and is classified as Critically Endangered. Here, we report a genome assembly of P. pselaphon, constructed using Nanopore long reads …
Read PDFSequencing-based surveillance can enable rapid and sensitive detection of environmental pathogens. The Oslofjord inlet is relatively narrow and is exposed to substantial human activity, including occasional wastewater contamination. Restricted water exchange also allows for occasional …
Read PDFWe present the complete genome sequence of <i>Erwinia spp. str</i>. LJJL01, isolated from waste charcoal in Colorado, USA, using Oxford Nanopore sequencing. This sequence provides important insights into this bacterium's metabolic and catabolic robustness to …
Read PDFEmergomyces (Ajellomycetaceae, Onygenales) is a genus of dimorphic fungal pathogens that cause severe, opportunistic infections around the world. We used long-read nanopore sequencing to generate de novo genome assemblies followed by comparative analyses for the …
Read PDFWe present a high-quality, chromosome-scale genome assembly for the redlip blenny Ophioblennius macclurei (family Blenniidae). The assembly was generated using a combination of Oxford Nanopore long-read sequencing, Illumina short-read data, and Hi-C scaffolding technology. The …
Read PDFChikungunya virus (CHIKV) poses an increasing global public health threat, as evidenced by the significant 2025 Foshan outbreak in China. Rapid, whole-genome sequencing (WGS) is critical for outbreak response but is challenged by primer mismatches …
Read PDFIn agriculture, biosecurity, and human health, the rapid and accurate detection of pathogens and pests is crucial. Our study investigates the sensitivity and practicality of six guide RNA (gRNA) production methods for use in Nanopore …
Read PDFWe report the complete genome of calcite-precipitating <i>Cytobacillus kochii</i> strain ITBMC_116, isolated from limestone karst in Vietnam. Hybrid Illumina-Nanopore sequencing yielded a 4.83-Mbp chromosome and an 87.4-kbp plasmid. This resource supports studies of microbially induced …
Read PDFLong-read RNA sequencing (RNA-seq) technologies have revolutionized transcriptomic research by enabling the sequencing of full-length RNA molecules, thus providing a more accurate characterization of complex transcript isoforms than traditional short-read approaches. In this study, we …
Read PDF<h4>Objectives</h4>The fruiting body of Rigidoporus glomeratus is a traditional medicinal herb employed by indigenous ethnic communities in the Gaoligong Mountain region. This fungus is known to be rich in various metabolites, including polyphenols, polysaccharides, flavonoids, …
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