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<h4>Objective</h4>Glioma recurrence severely impacts patient prognosis, with current treatments showing limited efficacy. Traditional methods struggle to analyze recurrence mechanisms due to challenges in assessing tumor heterogeneity, spatial dynamics, and gene networks. Single-cell combined spatial transcriptomics …
Read PDF<h4>Background</h4>DNA methylation and chromatin accessibility are pivotal epigenetic regulators of gene expression and cellular identity, with significant implications in tumorigenesis and progression. Current single-cell multi-omics methods are limited in throughput and sensitivity, hindering comprehensive biomarker …
Read PDF<h4>Background</h4>Immune checkpoint inhibitor-associated myocarditis (ICIAM) poses significant challenges for cancer immunotherapy, particularly regarding the safety and efficacy of immune checkpoint blockade (ICB) rechallenge.<h4>Methods</h4>The present study analyzed 23 cases of ICIAM by integrating longitudinal clinical data …
Read PDFColorectal cancer (CRC) progression is strongly shaped by the tumor microenvironment (TME), where complex interactions between epithelial, immune, and stromal cells orchestrate immune suppression and tumor evolution. To dissect these relationships at single-cell resolution, we …
Read PDFFunctional genomics screens in human induced pluripotent stem cells (hiPSCs) remain challenging despite their transformative potential. We developed iPS2-seq: an inducible, clone-aware screening platform that enables phenotype-agnostic, single-cell resolved dissection of loss-of-function effects in hiPSC …
Read PDFFoundation models (FMs) have been built to analyze single-cell data with different degrees of success. Here, we present scELMo (single-cell embedding from language models), a method for analyzing single-cell data with the help of large …
Read PDFAlzheimer's disease (AD) is a common neurodegenerative disorder; however, its molecular complexity remains poorly understood. Single-cell analysis can reveal the molecular changes in AD in different types of brain cells. In this study, we integrated …
Read PDF<h4>Background</h4>Single-cell RNA sequencing has emerged as a powerful approach to reveal cellular heterogeneity within biological systems. With the continuous advancement of high-throughput sequencing technologies, studies are generating vast amounts of complex data, posing a significant …
Read PDFThe study looked at how solid-state fermentation affects date palm leaves (DPL) and how it influences lamb performance, both with and without single-cell protein. DPL was fermented using three different strains of microbes. Using the …
Read PDFThe integration of single-cell multi-omics data provides a powerful approach for understanding the complex interplay between different molecular modalities, such as RNA expression, chromatin accessibility and protein abundance, measured through assays like scRNA-seq, scATAC-seq and …
Read PDFCondyloma acuminatum (CA), primarily caused by low-risk HPV6/11, is a benign proliferative disease that is difficult to cure and prone to recurrence. However, the molecular and immune mechanisms underlying relapse remain unclear. We combined metabolomic …
Read PDF<h4>Background</h4>Pyroptosis, an inflammatory form of programmed cell death, contributes to intestinal inflammation in inflammatory bowel disease (IBD), but the key cell types and regulatory genes remain unclear.<h4>Methods</h4>We analyzed single-cell RNA-seq data from intestinal mucosa to …
Read PDFCellular and molecular characterization of mammals with multi-chambered stomachs is crucial to our understanding of evolution in digestive and metabolic systems. Here, we generate single-cell transcriptomic atlases of 253,448 and 279,057 cells from 54 tissues …
Read PDF<h4>Background</h4>Head and Neck Squamous Cell Carcinomas (HNSCC) are the seventh most prevalent form of cancer and are associated with human papilloma virus infection (HPV-positive) or with tobacco and alcohol use (HPV-negative). HPV-negative HNSCCs have a …
Read PDFThe rapid advancement of multi-omics single-cell technologies has significantly enhanced our ability to investigate complex biological systems at unprecedented resolution. However, many existing analysis tools are complex, requiring substantial coding expertize, which can be a …
Read PDFZirconia (ZrO<sub>2</sub>) has become a promising alternative to titanium (Ti) for bone implants due to its excellent biocompatibility. Despite this, the osseointegration of ZrO<sub>2</sub> remains lower than that of Ti implants. However, the underlying biological …
Read PDFThe integration of high-throughput single-cell profiling technologies with RNA velocity analysis has enabled the reconstruction of dynamic cellular differentiation trajectories at unprecedented resolution. Despite these advances, current visualization techniques for RNA velocity are predominantly confined …
Read PDFThe accumulation of mitochondrial DNA (mtDNA) deletion mutants in post-mitotic cells is a hallmark of mammalian ageing and a key contributor to tissue decline in skeletal muscle and neurons. A transcription-coupled replication model predicts that …
Read PDFElucidating the mechanisms underlying vascular injury and repair may guide development of therapies against vascular cognitive impairment and dementia (VD). We employed single-cell RNA sequencing to map cell populations and explore vascular responses in mouse …
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