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Following thymic egress, CD8<sup>+</sup> T cells undergo post-thymic maturation to transition from recent thymic emigrants (RTEs) to mature naive T cells. Although RTEs are phenotypically and functionally distinct from mature naive CD8<sup>+</sup> T cells, most …
Read PDFThe pons and medulla, key regions of the hindbrain within the brainstem, regulate a wide range of complex behaviors, ranging from motor control to autonomic regulation and reflexes. As the central hub of neural projections …
Read PDFBACKGROUND: Breast cancer is one of the most prevalent malignant tumors among women, with its metastasis serving as a leading cause of mortality in affected patients. Recent studies have increasingly highlighted the significant role of …
Read PDFSingle-cell RNA sequencing (scRNA-seq) provides an instantaneous snapshot of the transcriptional state of a cell, which results from the simultaneous activity of many cellular processes. In this issue of Cell Genomics, Chen et al.<sup>1</sup> describe …
Read PDFThiols serve indispensable biochemical functions across catalysis, redox homeostasis and energy metabolism. However, profiling multiple thiols at the single-cell level remains challenging due to their trace amount and susceptibility to oxidation. Herein, we report an …
Read PDFAdeno-associated viral (AAV) vectors are rapidly advancing as gene therapies for retinal diseases, but gene therapy-associated uveitis (GTAU) limits their broader application. We assessed the ocular immune response to subretinal AAV gene therapy in two …
Read PDFProtein prenylation is a widespread post-translational modification (PTM) that regulates membrane association and signaling; dysregulation of this process leads to a variety of diseases. Metabolic labeling with probes containing bioorthogonal functionality has revolutionized the study …
Read PDFSystematic whole-protein screening and comprehensive profiling of antigen-specific CD4<sup>+</sup> T cells are crucial for advancing vaccine design and cancer immunotherapies, yet remain technically challenging. Here, we present a high-throughput platform that utilizes large-scale class II …
Read PDFHepatocellular carcinoma (HCC) represents one of the most prevalent malignancies worldwide and poses a critical public health challenge due to difficulties in early diagnosis, therapy resistance, and high mortality rates. The complex tumor microenvironment (TME) …
Read PDFMantle cell lymphoma (MCL) is a B-cell malignancy with a chronically relapsing clinical course and pronounced genetic heterogeneity. To investigate the clonal dynamics underlying early disease relapse, we performed single-cell RNA sequencing of paired tumor …
Read PDFSingle-cell technologies have revolutionized our ability to interrogate biological systems at unprecedented resolution, revealing complex cellular heterogeneity and dynamic processes that underlie development, disease, and immune responses. However, the high dimensionality and nonlinear structure of …
Read PDF<h4>Background</h4>Microvascular invasion (MVI) is a critical determinant of early recurrence and poor prognosis in hepatocellular carcinoma (HCC). The cellular and molecular mechanisms driving MVI, particularly the role of endothelial heterogeneity, remain incompletely understood.<h4>Methods</h4>We integrated single-cell …
Read PDFDisulfidptosis, a newly identified form of programmed cell death, has emerged as a promising therapeutic target for tumors. However, the current research in this field is hindered by the absence of precise and quantifiable biomarkers …
Read PDFUnderstanding cell state transitions and their governing regulatory mechanisms remains one of the fundamental questions in biology. We develop a computational method, state transition inference using cross-cell correlations (STICCC), for predicting reversible and irreversible cell …
Read PDFAdipose tissue plasticity and functional heterogeneity play a central role in maintaining energy homeostasis, and their malfunction leads to metabolic disorders such as obesity, diabetes, and cardiometabolic disease. Rapid, single-cell metabolic imaging of intact fat …
Read PDFImmune checkpoint blockade has shown benefit in some Triple-negative breast cancer (TNBC) patients, but responses are variable. BRCA1-mutated TNBC represents a biologically distinct subgroup, potentially differing in immunogenicity and immunotherapy responsiveness. However, immune microenvironment differences …
Read PDF<h4>Introduction</h4>The formation and homeostatic maintenance of cranial sutures rely on cellular activities within the suture mesenchyme. However, how mesenchymal stem/stromal cells (MSCs) rapidly and extensively contribute to suture and cranial development remains insufficiently explored.<h4>Methods</h4>We integrated …
Read PDF<h4>Background</h4>Atopic dermatitis (AD), a common chronic inflammatory skin disease, has been extensively studied using single-cell genomics. However, keratinocytes, as key effector cells in AD, have underlying mechanisms remain incompletely understood and require further investigation.<h4>Methods</h4>We integrated …
Read PDFThe growing global protein demand and environmental concerns from conventional animal agriculture have driven the exploration of sustainable alternative protein sources. Single-cell proteins (SCPs) from microbial fermentation offer a promising solution. This study comprehensively evaluated …
Read PDFRegenerative repair of injuries and degenerative diseases in the musculoskeletal system remains a paramount clinical challenge, as conventional single-modal technologies fail to recapitulate the intricate structural and functional complexity of native musculoskeletal tissues. In recent …
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