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Heterogeneity of Microglia in Ischemic Stroke from the Perspective of Single-Cell RNA Sequencing: Subset Characteristics, Mechanisms and Therapeutic Potential
Ischemic stroke (IS), a predominant cerebrovascular disorder contributing to global disability and mortality, characterizes by a complex, multi-tiered cascade of pathological processes. As the primary innate immune cells within the central nervous system (CNS), microglia …
Read PDFSingle-cell and bulk transcriptomic analyses identify B-cell senescence-associated biomarkers in papillary thyroid carcinoma
<h4>Background</h4>Papillary thyroid carcinoma (PTC) exhibits marked clinical heterogeneity that cannot be fully explained by tumor-intrinsic alterations alone. Emerging evidence suggests that immune cell senescence, particularly within B-cell compartments, may critically reshape the tumor immune microenvironment …
Read PDFSystematic Comparison of Droplet-Based and Microwell-Based Platforms for Comprehensive Single-Cell Transcriptomic Analysis in Clinical Samples
Single-cell RNA sequencing (scRNA-seq) is widely utilized in tumor research. However, platform-specific technical biases may impact data interpretation. This study compared the performance of droplet- and microwell-based scRNA-seq platforms in the analysis of clinical samples. …
Read PDFIn Situ Quantification of Bacterial Surface Charge at the Single-Cell Level for Modeling Transport under Electric Fields
Locally enhanced electric field treatment (LEEFT) inactivates bacteria via charge-dependent transport into localized high-field regions, requiring resolution of surface-charge heterogeneity rather than population-averaged zeta potentials. Here, we develop a high-throughput single-cell tracking platform to quantify …
Read PDFMolecular mechanisms underlying psoriasis and depression: an integrated analysis using mendelian randomization, transcriptomics, and single-cell sequencing
<h4>Background</h4>Psoriasis, an immune-mediated systemic inflammatory disease affecting skin, vessels, and joints, often co-occurs with depression. Routine depression screening is vital, as mood disorders link to inflammation, visible lesions, and functional limitations.<h4>Methods</h4>The study integrated Mendelian randomization …
Read PDFSingle-cell transcriptomic profiling of C. elegans Q neuroblast lineage during migration and differentiation
Proper migration and differentiation of neuroblasts into neurons are essential for the development of a healthy nervous system. In this context, the asymmetrical migration of Caenorhabditis elegans Q neuroblasts provides a powerful model for studying …
Read PDFSingle-cell profiling defines the cellular landscape of the urinary bladder: a scoping review
<h4>Background</h4>The urinary bladder contains a heterogeneous cell population. Multiple studies utilizing single-cell RNA sequencing techniques have uncovered the complex transcriptomic profile of cells in the healthy bladder.<h4>Objective</h4>The present study aims to map existing evidence on …
Read PDFSurvival of ampicillin-treated uropathogenic Escherichia coli is independent of single-cell growth rates
The refractoriness of persistent infections to antibiotics necessitates lengthy treatment regimens to prevent therapeutic failures and relapses. Persistence has been attributed to entry of a small fraction of bacterial cells into a slowly growing or …
Read PDFA single-cell atlas of RNA alternative splicing in the glioma-immune ecosystem
<h4>Background</h4>Single-cell analysis has refined our understanding of cellular heterogeneity in glioma, yet RNA alternative splicing, a critical layer of transcriptome regulation, remains underexplored at single-cell resolution. Here, we present a pan-glioma single-cell alternative splicing analysis …
Read PDFSingle-cell transcriptomic profiling combined with Mendelian randomization illuminates molecular drivers of bladder cancer
Bladder cancer exhibits marked cellular heterogeneity, which complicates the identification of molecular drivers with both functional and genetic relevance. The link between genetic susceptibility and transcriptional activity in tumorigenesis remains incompletely understood. Single-cell transcriptomes of …
Read PDFCarbon Dioxide Fixation Cascade for the Production of Single-Cell Protein by <i>Clostridium ljungdahlii</i> and <i>Yarrowia lipolytica</i>
<i>Clostridium</i> species and yeast single-cell protein (SCP), produced via industrial fermentation, offer a sustainable, high-protein alternative to conventional sources while advancing circular economy goals through CO<sub>2</sub> valorization. Here, we developed an integrated two-stage bioprocess for …
Read PDFSingle-Cell Transcriptomic Profiling Uncovers a Metastasis-Associated MUCL3<sup>+</sup> Signet-Ring Cell Subpopulation in Gastric Cancer
<b>Background</b>: Gastric signet-ring cell carcinoma (GSRCC) is an aggressive gastric cancer subtype with abundant mucin production and high metastatic propensity. However, scarcity of specific biomarkers has impeded clinical diagnosis and mechanistic research. This study systematically …
Read PDFSingle-cell transcriptome profiling reveals the heterogeneity of ossification of the posterior longitudinal ligament (OPLL) and its immune microenvironment
<h4>Background</h4>Ossification of the posterior longitudinal ligament (OPLL) is a heterotopic ossification process of the posterior longitudinal ligament (PLL) that can compress the spinal cord and nerve roots, yet its cellular heterogeneity and pathogenesis remain unclear.<h4>Methods</h4>We …
Read PDFSingle-cell RNA sequencing and integrated bioinformatics reveal new mitochondrial biomarkers in sarcopenia
<h4>Background</h4>Sarcopenia, characterized by age-related skeletal muscle loss and dysfunction, affects approximately 10% of adults over 60 years worldwide. Current diagnostic methods often detect sarcopenia only after substantial muscle deterioration has occurred, highlighting the critical need …
Read PDFIntegrated Single-Cell and Bulk RNA Sequencing Identifies Macrophage Heterogeneity and Mitophagy-Related Biomarkers in Idiopathic Pulmonary Fibrosis
Mitophagy clears damaged mitochondria and maintains normal macrophage function. Clarifying the associations between idiopathic pulmonary fibrosis (IPF), macrophages, and mitophagy is crucial for early diagnosis and clinical management. Core macrophage subsets were identified as M2 …
Read PDFA novel paradigm for single-cell annotation in stem cell research
Stem cell-derived models are a powerful tool for studying biology and are increasingly paving the way for cell-based therapies. However, understanding precisely which cell types are present and how closely they recapitulate in vivo cells …
Read PDFPeripheral and central immune features in insomnia: Integrative bulk and single-cell transcriptomic analyses reveal core hub genes and candidate compounds
Insomnia is closely associated with immune dysregulation, yet the overall pattern of peripheral-central immune disequilibrium and its underlying molecular basis remains incompletely understood. To characterize the peripheral-central immune features associated with insomnia, identify key immune …
Read PDFSingle-Cell Sequencing of a Bile Sample From an Acute Cholecystitis Patient
Single-cell sequencing is a novel approach to genome sequencing of clinical samples. However, there are only few studies using single-cell sequencing of genomes for bacterial infections. A 71-year-old woman presented to the emergency department with …
Read PDFSingle-cell epigenetic and transcriptomic states across the continuum of monoclonal B cell lymphocytosis to chronic lymphocytic leukemia
<h4>Background</h4>Chronic lymphocytic leukemia (CLL) develops from physiologic B cells through low- and high-count monoclonal B cell lymphocytosis (LC-/HC-MBL). The timing and nature of early B cell expansion and molecular evolution remain unclear, limiting prediction of …
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