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A bacterial isolate capable of utilizing calcium oxalate as its sole carbon source was isolated from desert soil to explore the role of oxalotrophy in drylands. Complete genomic sequencing and phylogenomic analysis identified the organism …
Read PDFTwo near-complete genomes of genotype II African swine fever viruses (ASFV) recovered from domestic pigs in Mbozi district, Tanzania, in 2017 were generated using tiled amplicon Oxford nanopore sequencing. These two ASFV genomes described in …
Read PDFParadombeya sinensis is an ecologically and phylogenetically valuable tree species endemic to southwestern China, yet its genomic resources remain underdeveloped. Here, we present a haplotype-resolved, chromosome-scale genome assembly to address this gap. The assembly spans …
Read PDFHEK293 is a preferred cellular platform to produce viral vectors including adeno-associated viruses (AAV). However, HEK293 cells were shown to be genomically unstable and many HEK293 cell lines having distinct genotypes and phenotypes have been …
Read PDFThis study identified and characterised three Genomoviruses during a circular DNA-enriched sequencing project aimed at assessing the evolution of Cassava mosaic begomoviruses in Nigeria. Using a combination of rolling circle amplification, Oxford Nanopore Sequencing and …
Read PDF<i>Geobacillus stearothermophilus</i> strain CS7089 was isolated as a thermophilic contaminant from commercial agar used to produce microbiological growth media. We present the 3,555,170 bp genome of this organism and report on its basic characteristics, as …
Read PDFAmong the various genotypes of the human papillomavirus (HPV), the HPV16 genotype is the most oncogenic by far, and some (sub)lineages may be associated with an increased risk of cancer. Thus, characterizing the full genome …
Read PDF<h4>Background</h4>Sanger sequencing remains the gold standard for characterizing genetic variants in short DNA fragments (<700 bp). However, the increasing demand for short TATs and high sensitivities in variant detection, particularly in oncohematology, is driving the …
Read PDF<i>Campylobacter</i>, a microaerophilic, Gram-negative spirillum, has been isolated from both animal and human specimens; however, most identified species are of animal origin. Here, we report the complete genome sequence of a new <i>Campylobacter</i> species, consisting …
Read PDFNipah virus (NiV) is a deadly zoonotic pathogen in Southeast Asia causing severe respiratory and encephalitis symptoms with a high fatality rate. Whole-genome sequencing (WGS) is crucial for tracking transmission, conducting epidemiological analyses, and understanding …
Read PDFSpinifex sericeus (beach spinifex) is a perennial, dioecious grass native to the coastal ecosystem of Oceania that has an important role in dune stability. We present a near telomere-to-telomere, haplotype-resolved nuclear genome and complete organelle …
Read PDFDuring the COVID-19 pandemic, next-generation sequencing (NGS) has been instrumental for public health laboratories in tracking severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) mutations and informing responses. Illumina systems and Oxford Nanopore Technologies (ONT) have …
Read PDFThe placenta is critical for fetal development and mediates effects of pregnancy complications on offspring metabolic health yet remains poorly characterized in genomic studies. Existing transcriptomic analyses rely on adult tissue reference annotations, overlooking developmentally …
Read PDFProtein-protein interactions (PPI) play a key role in host-pathogens interaction studies, as proteins are essential to many cellular mechanisms. The yeast two-hybrid (Y2H) approach is a well-established method for high-throughput PPI screening and mapping of …
Read PDF<i>Phlyctema vagabunda</i> is a plant pathogenic fungus widespread in Europe and North America that causes severe damages to different crop species. This announcement reports the genome sequence of <i>P. vagabunda</i> 19EL15 strain, associated with bull's …
Read PDF<h4>Background</h4>The gut microbiome possesses substantial genetic diversity that supports microbial adaptation, but the genomic variation patterns across its prokaryotic and viral populations remain incompletely characterized.<h4>Results</h4>Through integrated metagenomic and metatranscriptomic analysis of ten indigenous chicken breeds …
Read PDF<h4>Purpose</h4>This study aimed to comprehensively analyze the global landscape, trends, and research focus of nanopore sequencing technology in the field of pathogenic microorganism diagnosis using bibliometric analysis.<h4>Methods</h4>Literature published between January 2014, and December 2024, was …
Read PDFPlasmid construction is central to life science research, and sequence verification is arguably its costliest step. Long-read sequencing has emerged as a competitor to Sanger sequencing, with the principal benefit that whole plasmids can be …
Read PDFTrypanosoma lainsoni, Trypanosoma platydactyli, and Trypanosoma scelopori are kinetoplastid flagellates belonging to the LSRM clade of the genus Trypanosoma. These parasites have a unique mitochondrial DNA structure, the kinetoplast, comprising catenated maxicircles and minicircles. However, …
Read PDFCowpea (<i>Vigna unguiculata</i>) is a versatile legume crop providing a critical source of grain, vegetable and forage globally. Cultivated cowpea is classified into two main subspecies, subsp. <i>sesquipedalis</i> for fresh-pod vegetable and subsp. <i>unguiculata</i> for …
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