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Long COVID or Post-Acute Sequelae of SARS-CoV-2 Infection (PASC), marked by persistent symptoms lasting weeks to months after acute SARS-CoV-2 infection, affects multiple organ systems including the respiratory, cardiovascular, neurological, gastrointestinal, and renal systems. These …
Read PDFBioinformatics plays a crucial role in bacterial genomics and metagenomics research, transforming our understanding of microbial communities and their functions [...].
Read PDF<i>Campylobacter bilis</i> has been reported as a second causative agent of spotty liver disease, which leads to liver necrosis, increased mortality, and decreased egg production in laying hens. Here, we present the first two complete …
Read PDFHere, we report the resequencing, assembly, and annotation of two actinomycete genomes containing abyssomicin gene clusters. <i>Kutzneria buriramensis</i> DSM 45791 with a circular chromosome of 11,681,598 bp and 4 circular plasmids (14,175-207,548 bp) and <i>Streptomyces</i> …
Read PDFMulti-kilobase knock-ins (KIs) are a necessary, yet challenging type of genome editing to create and characterize in cell lines and animals. The combination of rAAV donor transduction and electroporation of single-cell mouse embryos with Cas9/gRNA …
Read PDFReconstructing exact haplotypes is important when sequencing a mixture of similar sequences. Long-read sequencing can connect distant alleles to disentangle similar haplotypes, but handling sequencing errors requires specialized techniques. Here, we present <i>devider</i>, an algorithm …
Read PDF<h4>Background</h4>Hao-Fountain syndrome (HAFOUS) is a rare neurodevelopmental disorder caused by pathogenic variants in the <i>USP7</i> gene. This condition is associated with a distinct DNA methylation episignature that aids its diagnosis. While microarray-based methods have traditionally …
Read PDFThe assembly of insect mitochondrial genomes remains challenging in certain taxa due to extreme AT biases and long repetitive regions. The family Diaspididae (Hemiptera: Coccomorpha) exemplifies this difficulty; despite more than 2,700 described species worldwide, …
Read PDFAs a protandrous hermaphroditic fish species with natural sex change from male to female, Asian seabass (Lates calcarifer) represents an attractive model for studying sequential hermaphroditism. In this study, we constructed the first telomere-to-telomere (T2T) …
Read PDFShort-read metabarcoding analysis is the gold standard for accessing partial 16S and ITS genes with high read quality. With the advent of long-read sequencing, the amplification of full-length target genes is possible, but with low …
Read PDFThe Cape Floristic Region, a biodiversity hotspot in South Africa, is characterised by acidic, nutrient-poor soils and distinctive fynbos vegetation. Despite the ecological importance and metabolic versatility of Acidobacteriota, their diversity and functional roles in …
Read PDFHere, we report the complete genome sequence of <i>Pseudomonas</i> sp. KK18, isolated from the Antarctic Ross Sea sediment. The isolate has a genome size of 7.3 Mb, 61% GC content, 100% completeness, and 0.36% contamination. …
Read PDF<i>Mannheimia haemolytica</i> is a pathobiont bacterium of the upper respiratory tract in cattle and is associated with acute bovine respiratory disease (BRD). Despite research into bacterial pathogens and their associations with BRD, little is known …
Read PDF<b>Background/Objectives:</b> The emergence of antimicrobial-resistant (AMR) pathogens in aquaculture ecosystems poses a significant risk to both food security and human health. <i>Shewanella</i> species are recognized as significant AMR reservoirs, yet their prevalence and resistance mechanisms …
Read PDFRestriction-modification (RM) systems are widespread defense mechanisms in prokaryotes that protect the host from potentially harmful foreign DNA. They typically consist of a DNA methyltransferase (MTase), which methylates the host genome at an adenine (6 …
Read PDFThe growing resistance of <i>Enterobacterales</i> to last-resort antibiotics such as carbapenems puts a significant burden on healthcare systems, also due to plasmids driving a rapid spread of carbapenem resistance. We here evaluate the use of …
Read PDFUropathogenic <i>Escherichia coli</i> (UPEC) is a leading cause of urinary tract infections (UTIs) in companion animals. This study characterized 42 UPEC isolates recovered from dogs and cats at the University of Florida, College of Veterinary …
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