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<h4>Background</h4>The human endometrium is a regenerative tissue relying on stem/progenitor cells. Endometrial mesenchymal stem cells (eMSCs) are typically enriched using perivascular markers like CD140b and CD146. However, the identity of more primitive and quiescent eMSC …
Read PDFProteins that impact phenotype and disease are often approximated by RNA expression, which poorly infers protein abundance. We developed DeepGxP, a deep-learning model trained on The Cancer Genome Atlas pan-cancer data, to predict protein abundance …
Read PDF<b>Background/Objectives</b>: Sex differences influence skin physiology, immune regulation, and disease susceptibility, but the cellular organization of sex-biased transcriptional programs in healthy human skin remains incompletely defined. We aimed to define sex-associated differences in cellular composition …
Read PDFNeural stem cells (NSCs) are self-renewing, multipotent cells capable of differentiating into neurons, astrocytes, and oligodendrocytes. NSCs reside in specific brain niches: the ventricular zone (VZ), the subventricular zone (SVZ) and the subgranular zone of …
Read PDFPeriodontitis is a chronic inflammatory disease affecting tooth-supporting tissues. Beyond microbial dysbiosis, host metabolic regulation plays a critical role. This study identified ornithine aminotransferase (OAT), a mitochondrial enzyme in amino acid metabolism, as associated with …
Read PDFRecent years have seen a rise of single-cell proteomics by data-independent acquisition mass spectrometry (DIA MS). While diverse data analysis strategies have been reported in literature, their impact on the outcome of single-cell proteomic experiments …
Read PDFIn recurrent spontaneous abortion (RSA), impaired decidualization due to decidual stromal cell (DS) apoptosis or pyroptosis disrupts pregnancy maintenance. RNA-binding proteins (RBPs) are crucial regulators of gene expression in reproductive disorders. However, the comprehensive profile …
Read PDF<h4>Background</h4>Acupuncture has been proposed as a therapeutic intervention for stroke recovery, yet the underlying molecular mechanisms remain poorly understood.<h4>Method</h4>In this study, we used a mouse model of hemorrhagic stroke induced by autologous blood injection to …
Read PDFGenetic interactions (GIs) drive carcinogenesis and treatment resistance via non-additive phenotypic effects between genes. Traditional bulk-based methods fail to capture cell-type-specific interactions in heterogeneous tumors like lung adenocarcinoma (LUAD), limiting precision oncology. Resolving cell-type-specific GIs …
Read PDF<h4>Background</h4>The pathogenesis of HSPCs impairment in aplastic anemia (AA) remains unclear. We focused on CD8<sup>+</sup> T cells and investigated platelets, CD4<sup>+</sup> T cells, macrophages, and mitochondrial energy metabolism, aiming to elucidate the cellular atlas changes …
Read PDF<h4>Background</h4>Prostate cancer (PCa) is the second most common malignancy in men worldwide. Although radical prostatectomy effectively controls tumor progression, it often causes complications such as urinary incontinence and sexual dysfunction. Irreversible electroporation (IRE), a non-thermal …
Read PDFBACKGROUND: Gastric cancer (GC) characterized by profound cellular heterogeneity, with the diversity and interactions of cells within the tumor microenvironment (TME) playing critical roles in tumorigenesis, metastasis, and therapeutic response. However, the underlying mechanisms remain …
Read PDF<h4>Background</h4>Advancements in single-cell RNA sequencing have enabled the analysis of millions of cells, but integrating such data across samples and methods while mitigating batch effects remains challenging. Deep learning approaches address this by learning biologically …
Read PDF<h4>Introduction</h4>Plant development is shaped by environmental conditions, and its adaptation to climate change is crucial for biodiversity conservation. The extreme climate of the Qinghai-Tibet Plateau makes it an ideal system for studying plant adaptive strategies. …
Read PDFIn the brain, endothelial cell (EC) subtypes characterized by blood-brain barrier (BBB) properties or fenestrated pores form essential brain-blood interfaces and exhibit markedly distinct permeability. The choroid plexus (CP) establishes fenestrated vasculature lacking the BBB …
Read PDFWhile single-cell RNA sequencing has advanced our understanding of cell fate, identifying molecular hallmarks of potency-a cell's ability to differentiate into other cell types-remains a challenge. Here we introduce CytoTRACE 2, an interpretable deep learning …
Read PDFThe progression of lung adenocarcinoma (LUAD) is influenced by polyamine metabolism, which modulates antitumor immunity, although the underlying mechanisms remain unclear. The present study investigates the role of polyamine metabolism-related genes (PMRGs) in LUAD using …
Read PDF<h4>Background</h4>The high dimensionality of data in single cell transcriptomics (scRNAseq) requires investigators to choose subsets of genes ("feature selection") for downstream analysis (e.g., unsupervised cell clustering). The evaluation of different approaches to feature selection is …
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