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<h4>Background</h4>Basophils are implicated in various diseases including allergies, but a comprehensive single-cell characterization of human basophils has yet to be performed. Here, we aimed to generate a single-cell omics-based reference resource of circulating human basophils, …
Read PDFWild carnivores can harbor pathogens affecting wildlife conservation and domestic animal health. This study surveyed major viral pathogens in free-ranging wolves, red foxes, stone martens, and Eurasian badgers in Northwestern Italy. Duodenal samples from 140 …
Read PDFOff-white or yellowish shoots are common in tea plants (<i>Camellia sinensis</i> L.), and such albino variations are often accompanied by metabolic reprogramming, including increased contents of amino acids and lower levels of polyphenols. Nonetheless, the …
Read PDF<i>Rhodotorula pacifica</i> INDKK, an oleaginous red yeast isolated from mangrove forest soil, efficiently metabolizes biomass hydrolysates and accumulates lipids of more than 60% of its dry weight. Hybrid <i>de novo</i> genome assembly (Illumina and Oxford …
Read PDF<h4>Background</h4>Colorectal cancer (CRC) is the third most common cancer globally. Alternative splicing contributes significantly to CRC tumorigenesis through aberrant transcript generation. However, the regulatory influence of RNA modifications on splicing remains poorly understood, largely due …
Read PDFLong-read amplicon profiling through read classification limits phylogenetic analysis of amplicons while community analysis of multicopy genes, relying on unique molecular identifier (UMI) corrections, often demands deep sequencing. To address this, we present a long …
Read PDFBiodiversity monitoring using metabarcoding is now widely used as a routine environmental management tool. However, despite the rapid advancement of third-generation high-throughput sequencing platforms, there are limited studies assessing the most suitable tools and approaches …
Read PDFDuring the COVID-19 pandemic, stringent public health measures led to historically low influenza activity in Hong Kong. However, after these interventions were relaxed in 2023, Influenza A viruses (IAV), including A(H1N1)pdm09 and A(H3N2), rapidly resurfaced. …
Read PDFThird-generation sequencing technologies have revolutionized the study of epigenetic characteristics in human diseases, with Oxford Nanopore Technologies (ONT) at the forefront of long-read sequencing. ONT has made rapid improvements in flow cell designs, which greatly …
Read PDFEwing sarcoma is a rare and aggressive cancer of the bone and soft tissues primarily affecting children and young adults. Prognosis for patients with metastatic or recurrent disease remains poor despite intensive multimodal therapy, highlighting …
Read PDFThe endangered mountain gorilla, Gorilla beringei beringei, faces numerous threats to its survival, highlighting the urgent need for genomic resources to aid conservation efforts. Here, we present a near telomere-to-telomere, haplotype-phased reference genome assembly for …
Read PDF<h4>Objective</h4>This study presents the whole genome sequence of Staphylococcus aureus strain 37M isolated from cows with subclinical mastitis in Kiruhura district, Uganda. The objective was to characterize the genome to understand the isolate's resistance and …
Read PDF<h4>Introduction</h4><i>Synchytrium endobioticum</i>, the biotrophic pathogen causing potato wart, poses persistent challenges due to its long-term soil survival and quarantine status. Biological control agents (BCAs) offer a promising avenue for sustainable management, yet the ecological context …
Read PDFInfluenza A virus (IAV) remains a major health concern in both humans and animals, with pigs serving as key reservoirs for generating novel reassortant viruses with pandemic potential. Respiratory microbiome alterations during infection may facilitate …
Read PDF<b>Background/Objectives</b>: Rapid and accurate pathogen identification and antimicrobial susceptibility testing (AST) are critical for the proper management of patients with bloodstream infection (BSI). Real-time whole-genome sequencing (WGS) represents an attractive opportunity for exhaustive pathogen identification …
Read PDFThe complete genome of <i>Lactococcus lactis</i> isolate S11-599 is presented, recovered from the brain of a silver carp (<i>Hypophthalmichthys molitrix</i>) during a fish mortality event affecting invasive carp in the Mississippi River.
Read PDFThe selection of a reference sequence in genome analysis is critical, as it serves as the foundation for all downstream analyses. Recently, the pangenome graph has been proposed as a data model that incorporates haplotypes …
Read PDF<b>Background:</b> Medulloblastoma is the childhood tumor with the highest morbidity and mortality worldwide. This type of cancer is characterized by a high degree of heterogeneity that gives rise to different molecular groups with disparities in …
Read PDFHigh pathogenicity avian influenza (HPAI) H5 viruses of the Goose/Guangdong lineage continue to diversify through recurrent reassortment, sustaining an evolving threat to animal health and transboundary disease control in Europe. While multiple incursions of HPAI …
Read PDF<h4>Background</h4>The human gut virome is a fundamental yet understudied component of the intestinal microbiome. However, its taxonomic composition and functional potential in Latin American populations remain poorly understood, particularly under clinical stressors such as hospitalization …
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