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Cellular heterogeneity is a fundamental facet of cell biology, influencing cellular signaling, metabolism, and gene regulation. Its accurate quantification requires measurements at the single-cell level. Most high-throughput single-cell technologies provide only a snapshot of cellular …
Read PDFSingle-cell RNA sequencing (scRNA-Seq) enables advanced analysis of cellular heterogeneity; however, due to data sparsity and noise, analyzing single genes can be challenging. While pathway activity scoring offers a robust alternative to standard single-gene analysis, …
Read PDFChimeric antigen receptor T cell (CAR T) immunotherapies have transformed cancer treatment. These therapies typically involve lentiviral vector-mediated CAR gene integration, followed by reinfusion into patients, necessitating rigorous quality control. Critical parameters, including transduction efficiency …
Read PDFRobust perfect adaptation (RPA), whereby a consistent output level is maintained even after a disturbance, is a highly desired feature in biological systems. This property can be achieved at the population average level by combining …
Read PDFThe tumor microenvironment plays a critical role in therapeutic resistance in diffuse large B-cell lymphoma (DLBCL), yet actionable targets within the immune cells remain largely undefined. Through integrative multiomics profiling, we identified tumor necrosis factor …
Read PDFThe tumor microenvironment (TME) has a profound influence on the progression of lung adenocarcinoma (LUAD) and its response to therapy. We identified a tumor-promoting communication network based on single-cell RNA sequencing. This shows an SPP1 …
Read PDFRegulatory T cells (Treg) play immunosuppressive roles in lung cancer and are key biomarkers in cancer immunology. Using scRNA-seq and stereo-seq, we aimed to identify a novel circulating Treg subtype in non-small cell lung cancer …
Read PDFDifferential detergent fractionation (DDF) enables compartment-specific lysis, offering a strategy to analyze cytoplasmic proteins while preserving the nucleus for downstream assays. However, while this method facilitates sub-cellular resolution, current single-cell approaches using DDF remain limited …
Read PDFThe quantum dot (QD)-enhanced optical biosensors are very sensitive tools for analyzing single cells. Heavy metal-free alternatives, such as indium phosphide and carbon-based QDs, address safety concerns while achieving high quantum efficiency. Photonic enhancement techniques …
Read PDFAccurate deconvolution of bulk and spatial transcriptomes is essential for studying tissue architecture and disease, yet remains challenged by unmodeled differences in cellular RNA content and cross-source heterogeneity. We introduce CSsingle, a unified deconvolution framework …
Read PDFTuberculous meningitis (TBM) is the most severe form of tuberculosis, with a fatality rate of 20% to 50% in treated individuals. Although corticosteroid therapy can increase survival in HIV-negative people with TBM, better antimicrobial and …
Read PDF<i>Salmonella</i> is a major foodborne pathogen that causes approximately 1.35 million infections annually in the US and remains a leading cause of poultry-associated foodborne illness. To improve chickens' resistance to this pathogen, it is important …
Read PDFWe previously demonstrated that blocking TGF-β with galunisertib, a safe, orally available small drug, reactivated latent SIV in vivo by shifting T cells toward a transitional effector phenotype. Here, we investigated the mechanisms underlying this …
Read PDFErythrocyte aging is a fundamental physiological phenomenon that involves significant structural and nanomechanical alterations of the cells' structure and function. Coupling optical, fluorescence, and Atomic Force Microscopy (AFM), we analyzed morphology, membrane roughness and nanomechanical …
Read PDF<h4>Introduction</h4>Kawasaki disease (KD) is an acute autoimmune vasculitis that predominantly affects children under 5 years of age. Although immune dysregulation is considered central to KD pathogenesis, the cellular heterogeneity and regulatory mechanisms underlying this process …
Read PDFPURPOSE: The complex tumor microenvironment (TME) in ovarian cancer (OC), particularly the role of macrophage clusters, plays a pivotal role in driving cancer progression and determining patient prognosis. Metabolic reprogramming is a hallmark of tumorigenesis …
Read PDFGaining insight into the circuit architecture of neural tissue significantly aids in the investigation of nervous system mechanisms. Since neural networks are constituted by connections between axons and dendrites derived from neurons, morphological analysis of …
Read PDFAging gradually impairs immune system function, yet its systemic features across immune organs remain poorly characterized in primates. Here, we perform single-cell transcriptomic profiling of bone marrow, spleen, mesenteric lymph nodes, and peripheral blood mononuclear …
Read PDFExpanding metabolomic profiling to the single-cell level can reveal metabolic heterogeneity and clinically relevant subpopulations, yet existing methods lack sensitivity and scale. To address this gap, in a recent issue of Cell, Delafiori and colleagues …
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