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Full text 2025

Reference-based chromosome-scale assembly of Japanese barley (Hordeum vulgare ssp. vulgare) cultivar Hayakiso 2

Tanaka T, Haraguchi Y, Todoroki T, et al.

Current advances in next-generation sequencing (NGS) technology and assembling programs permit construct chromosome-level genome assemblies in various plants. In contrast to resequencing, the genome sequences provide comprehensive annotation data useful for plant genetics and breeding. …

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Genome Assembly Nanopore Sequencing
Full text 2025

Beyond single references: pangenome graphs and the future of genomic medicine

Nyaga DM, Zaied RE, Silander OK, et al.

Genomic medicine relies on single reference genomes that miss crucial genetic diversity, creating diagnostic gaps that disproportionately affect underrepresented populations. Pangenome graphs, collections of diverse genomes represented as interconnected genetic paths, offer a powerful alternative …

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Genome Assembly Nanopore Sequencing Structural Variants
Full text 2025

SimGBS: a rapid method for simulating large-scale genotyping-by-sequencing data

Kang J, Hess MK, Dodds KG, et al.

BACKGROUND: Genotyping-by-sequencing (GBS) offers a cost-effective solution to access genomic information on many samples. The advent of GBS has promoted large scale genomic analyses, including linkage analysis, heritability estimation, inference of genetic relatedness and genome-wide …

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Bioinformatics