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Regulatory T cells (Treg) play immunosuppressive roles in lung cancer and are key biomarkers in cancer immunology. Using scRNA-seq and stereo-seq, we aimed to identify a novel circulating Treg subtype in non-small cell lung cancer …
Read PDFDifferential detergent fractionation (DDF) enables compartment-specific lysis, offering a strategy to analyze cytoplasmic proteins while preserving the nucleus for downstream assays. However, while this method facilitates sub-cellular resolution, current single-cell approaches using DDF remain limited …
Read PDFThe quantum dot (QD)-enhanced optical biosensors are very sensitive tools for analyzing single cells. Heavy metal-free alternatives, such as indium phosphide and carbon-based QDs, address safety concerns while achieving high quantum efficiency. Photonic enhancement techniques …
Read PDFAccurate deconvolution of bulk and spatial transcriptomes is essential for studying tissue architecture and disease, yet remains challenged by unmodeled differences in cellular RNA content and cross-source heterogeneity. We introduce CSsingle, a unified deconvolution framework …
Read PDFTuberculous meningitis (TBM) is the most severe form of tuberculosis, with a fatality rate of 20% to 50% in treated individuals. Although corticosteroid therapy can increase survival in HIV-negative people with TBM, better antimicrobial and …
Read PDFErythrocyte aging is a fundamental physiological phenomenon that involves significant structural and nanomechanical alterations of the cells' structure and function. Coupling optical, fluorescence, and Atomic Force Microscopy (AFM), we analyzed morphology, membrane roughness and nanomechanical …
Read PDF<h4>Introduction</h4>Kawasaki disease (KD) is an acute autoimmune vasculitis that predominantly affects children under 5 years of age. Although immune dysregulation is considered central to KD pathogenesis, the cellular heterogeneity and regulatory mechanisms underlying this process …
Read PDFGaining insight into the circuit architecture of neural tissue significantly aids in the investigation of nervous system mechanisms. Since neural networks are constituted by connections between axons and dendrites derived from neurons, morphological analysis of …
Read PDFAging gradually impairs immune system function, yet its systemic features across immune organs remain poorly characterized in primates. Here, we perform single-cell transcriptomic profiling of bone marrow, spleen, mesenteric lymph nodes, and peripheral blood mononuclear …
Read PDFExpanding metabolomic profiling to the single-cell level can reveal metabolic heterogeneity and clinically relevant subpopulations, yet existing methods lack sensitivity and scale. To address this gap, in a recent issue of Cell, Delafiori and colleagues …
Read PDFProteins that impact phenotype and disease are often approximated by RNA expression, which poorly infers protein abundance. We developed DeepGxP, a deep-learning model trained on The Cancer Genome Atlas pan-cancer data, to predict protein abundance …
Read PDFNeural stem cells (NSCs) are self-renewing, multipotent cells capable of differentiating into neurons, astrocytes, and oligodendrocytes. NSCs reside in specific brain niches: the ventricular zone (VZ), the subventricular zone (SVZ) and the subgranular zone of …
Read PDFRecent years have seen a rise of single-cell proteomics by data-independent acquisition mass spectrometry (DIA MS). While diverse data analysis strategies have been reported in literature, their impact on the outcome of single-cell proteomic experiments …
Read PDF<h4>Background</h4>Acupuncture has been proposed as a therapeutic intervention for stroke recovery, yet the underlying molecular mechanisms remain poorly understood.<h4>Method</h4>In this study, we used a mouse model of hemorrhagic stroke induced by autologous blood injection to …
Read PDFGenetic interactions (GIs) drive carcinogenesis and treatment resistance via non-additive phenotypic effects between genes. Traditional bulk-based methods fail to capture cell-type-specific interactions in heterogeneous tumors like lung adenocarcinoma (LUAD), limiting precision oncology. Resolving cell-type-specific GIs …
Read PDF<h4>Background</h4>The pathogenesis of HSPCs impairment in aplastic anemia (AA) remains unclear. We focused on CD8<sup>+</sup> T cells and investigated platelets, CD4<sup>+</sup> T cells, macrophages, and mitochondrial energy metabolism, aiming to elucidate the cellular atlas changes …
Read PDF<h4>Background</h4>Prostate cancer (PCa) is the second most common malignancy in men worldwide. Although radical prostatectomy effectively controls tumor progression, it often causes complications such as urinary incontinence and sexual dysfunction. Irreversible electroporation (IRE), a non-thermal …
Read PDF<h4>Background</h4>Advancements in single-cell RNA sequencing have enabled the analysis of millions of cells, but integrating such data across samples and methods while mitigating batch effects remains challenging. Deep learning approaches address this by learning biologically …
Read PDFIn the brain, endothelial cell (EC) subtypes characterized by blood-brain barrier (BBB) properties or fenestrated pores form essential brain-blood interfaces and exhibit markedly distinct permeability. The choroid plexus (CP) establishes fenestrated vasculature lacking the BBB …
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