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Techniques exist to isolate cells from fresh intestinal tissue; however, approaches for recovering viable cells from cryopreserved duodenal and colonic specimens are lacking. Here, we present a protocol for isolating cells from a single cryopreserved …
Read PDFOvarian cancer(OC) poses a significant clinical challenge due to its frequent peritoneal dissemination and development of chemotherapy resistance, contributing to poor patient outcomes. Chronic inflammation is a pivotal driver of disease initiation and progression. While …
Read PDF<h4>Background</h4>Neutrophils, which are polymorphonuclear leukocytes of the phagocytic system, serve as critical mediators of inflammation-induced injury and are implicated in various diseases. However, the role of neutrophils in primary Sjögren's syndrome (pSS) remains underexplored.<h4>Objectives</h4>Single-cell RNA …
Read PDFSingle-cell RNA sequencing data rely heavily on dimensionality reduction methods to uncover underlying structures and patterns. Nonlinear dimensionality-reduction methods such as uniform manifold approximation and t-distributed stochastic neighbor embedding have been routinely used for this …
Read PDFEndometriosis is a chronic hormone-responsive disorder linked to infertility, usually characterized by the presence of ectopic endometrium in the pelvis that disrupts local homeostasis. Advances in single-cell "omic" methods have revealed the remarkable cellular diversity …
Read PDFPeriodontitis (PD) and type 2 diabetes mellitus (DM) are bidirectionally associated through shared chronic inflammatory mechanisms. Although monocytes serve as central immune mediators in both pathologies, their functional heterogeneity and dynamic alterations in diabetic periodontitis …
Read PDFSingle-cell RNA-sequencing (scRNA-seq) can excavate cellular heterogeneity and distinguish different types of cells. Clustering cells into subpopulations is essential in analyzing scRNA-seq data as it can help subsequent downstream analysis. However, scRNA-seq data are high-dimensional, …
Read PDFWe present a microchannel-based adaptation of mass density-gradient centrifugation for the mass density measurement of cells or microparticles. The workflow consists of three basic steps: Microchannel filling, centrifugation, and microscopy. Microchannel filling and subsequent centrifugation …
Read PDFCell therapy has achieved a critical breakthrough through single-cell microgel technology. This miniaturized encapsulation platform enables precise microenvironment recapitulation, efficient targeted delivery, and tunable pericellular matrix control. Nevertheless, prevailing microfluidic and surface chemical engineering methodologies …
Read PDFTranslating high-resolution multiomics data into clinically actionable biomarkers is critical for overcoming therapeutic resistance and tumor heterogeneity in prostate adenocarcinoma (PRAD). To decode the complex immunosuppressive tumor microenvironment (TME) and identify robust prognostic targets, we …
Read PDFThe human placenta sustains pregnancy through intricate trophoblast lineage dynamics that are critical for fetal development and pregnancy success. While studies on protein-coding genes (PCGs) have advanced our understanding of placental biology, the regulatory roles …
Read PDFStudying cell-to-cell heterogeneity is essential to understand how unicellular organisms respond to stresses. We introduce a single-cell analysis framework that enables the study of intercellular heterogeneity of photosynthetic traits, particularly their interactions within individual cells …
Read PDF<h4>Background</h4>Migraine (MI), Ménière's disease (MD), and vestibular migraine (VM) share significant clinical and pathological similarities, particularly their link to neurological dysfunction and immune cell activity, though mechanisms remain poorly understood.<h4>Methods</h4>We utilized a single-cell RNA sequencing …
Read PDFSingle-cell three-dimensional genome sequencing (sc3DG-seq) reveals genome regulation and heterogeneity in various biological processes, but a universal analysis tool is lacking. Here we present STARK, a versatile toolkit for processing, quality control, and analysis of …
Read PDFMicrovessels within atherosclerotic plaques are crucially involved in disease progression. Here, we generated a transcriptomic atlas of human atherosclerosis at single-cell resolution, encompassing 17,367 vascular endothelial cells (VECs) from five scRNA-seq studies, and verified key …
Read PDFSunlight is a fundamental immune-modulating factor with implications for the pathogenesis of autoimmune diseases such as multiple sclerosis (MS). MS incidence and severity increase with latitude, historically attributed to diminished ultraviolet (UV) exposure and lower …
Read PDF<h4>Summary</h4>Cell population plots are visualizations showing cell population distributions in biological samples with single-cell data, traditionally shown with stacked bar charts. Here, we address issues with this approach, particularly its limited scalability with increasing number …
Read PDF<h4>Background</h4>Hepatocellular carcinoma (HCC) is one of the most common types of cancer globally. However, HCC features poor prognosis due to complex pathogenesis and limitations of therapeutic approaches.<h4>Method</h4>To improve the prognosis of HCC patients, we analyzed …
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