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768 results

Full text 2020

Long-read sequencing and de novo genome assembly of marine medaka (Oryzias melastigma)

Liang P, Saqib HSA, Ni X, et al.

<h4>Background</h4>Marine medaka (Oryzias melastigma) is considered as an important ecotoxicological indicator to study the biochemical, physiological and molecular responses of marine organisms towards increasing amount of pollutants in marine and estuarine waters.<h4>Results</h4>In this study, we …

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Genome Assembly Nanopore Sequencing Single-Cell
Full text 2021

Long-Read Sequencing Reveals Evolution and Acquisition of Antimicrobial Resistance and Virulence Genes in <i>Salmonella enterica</i>

Li C, Tyson GH, Hsu CH, et al.

<i>Salmonella enterica</i> is a significant and phylogenetically diverse zoonotic pathogen. To understand its genomic heterogeneity and antimicrobial resistance, we performed long-read sequencing on <i>Salmonella</i> isolated from retail meats and food animals. A collection of 134 …

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Genome Assembly Nanopore Sequencing
Full text 2022

Recovering individual haplotypes and a contiguous genome assembly from pooled long-read sequencing of the diamondback moth (Lepidoptera: Plutellidae)

Whiteford S, Van't Hof AE, Van't Hof AE, et al.

The assembly of divergent haplotypes using noisy long-read data presents a challenge to the reconstruction of haploid genome assemblies, due to overlapping distributions of technical sequencing error, intralocus genetic variation, and interlocus similarity within these …

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Bioinformatics Genome Assembly Nanopore Sequencing
Full text 2025

Chromosome-level genome assembly of the aphid Megoura crassicauda

Huang T, Cheng X, Wang B, et al.

Megoura crassicauda (Hemiptera: Aphididae) is a major pest species that inflicts significant damage on various legume crops worldwide, causing substantial global economic losses. In this study, we present a chromosome-scale genome assembly of M. crassicauda. …

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Genome Assembly Nanopore Sequencing
Full text 2025

The N-Terminal Domain of Tailspike Depolymerases Affects the Replication Efficiency of Synthetic <i>Klebsiella</i> Phages

Baykov IK, Mikhaylova EE, Miroshnikova AV, et al.

Bacteriophage receptor-binding proteins are often attached to the tail via a conserved N-terminal adapter/anchor domain, presumed to function independently from the distal receptor-binding/catalytic domain. Using synthetic phage technology, we demonstrated that the N-terminal domain in …

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Bioinformatics Genome Assembly
Full text 2025

A Chromosome-level genome assembly of the American bullfrog (Aquarana catesbeiana)

Zhang K, Zhang Y, Tian Y, et al.

The American bullfrog (Aquarana catesbeiana) is both an economically important aquaculture species and a globally distributed invasive organism with high environmental adaptability. In this study, we present a high-quality chromosome-level genome assembly for the species, …

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Genome Assembly Transcriptomics
Full text 2025

The genome sequence of the common hop, &lt;i&gt;Humulus lupulus&lt;/i&gt; L

Christenhusz MJM, Percival-Alwyn L, Hajdu K, et al.

We present a genome assembly from a female <i>Humulus lupulus</i> plant (Common hop, Hops; Streptophyta; Magnoliopsida; Rosales; Cannabaceae). The genome sequence has a total length of 2,488.10 megabases. Most of the assembly (99.39%) is scaffolded …

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Genome Assembly
Full text 2025

The genome sequence of the Bog Ant Fly, &lt;i&gt;Microdon myrmicae&lt;/i&gt; Schönrogge &lt;i&gt;et al&lt;/i&gt;., 2002

Wolton RJ, University of Oxford and Wytham Woods Genome Acquisition Lab, Darwin Tree of Life Barcoding collective, et al.

We present a genome assembly from a male specimen of <i>Microdon myrmicae</i> (Bog Ant Fly; Arthropoda; Insecta; Diptera; Syrphidae). The genome sequence has a total length of 1,366.18 megabases. Most of the assembly (99.91%) is …

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Genome Assembly
Full text 2026

Chromosome-level genome assembly of triploid Cyperus rotundus uncovers allelic expression patterns and herbicide resistance mechanisms

Wu L, Luo Z, Lu Y, et al.

Cyperus rotundus, a globally distributed and highly competitive weed, has evolved herbicide resistance, posing a significant challenge to sustainable agriculture; however, the lack of genomic resources has limited comprehensive investigations into its resistance mechanisms. Here, …

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Genome Assembly Transcriptomics
Full text 2025

Chromosome-level genome assembly of the autotetraploid yellow pitaya provides novel insights into evolution of trait patterning in pitaya species with different ploidy

Zaman QU, Raza A, Hui L, et al.

<h4>Background</h4>Yellow pitaya (Selenicereus megalanthus, 2n = 4x = 44) breeding remains severely hindered due to the lack of a reference genome.<h4>Results</h4>Here, we present a high-quality chromosome-level genome assembly of yellow pitaya using PacBio HiFi sequencing …

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Epigenetics Genome Assembly Structural Variants
Full text 2025

ERGA-BGE reference genome of &lt;i&gt;Lewinskya acuminata,&lt;/i&gt; a common epiphytic Mediterranean moss with disjunct populations in California and Ethiopia

Aguado-Ramsay P, Lara F, Draper I, et al.

The reference genome of <i>Lewinskya acuminata</i> (H. Philib.) F. Lara, Garilleti & Goffinet will enable phylogenomic, biogeographic, and evolutionary studies within the <i>Orthotrichaceae</i> and related bryophyte lineages at a depth previously inaccessible. This species of …

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Genome Assembly
Full text 2025

The genome sequence of Bottle Sedge, &lt;i&gt;Carex rostrata&lt;/i&gt; Stokes

Ruhsam M, Griffiths A, Royal Botanic Garden Edinburgh Genome Acquisition Lab, et al.

We present a genome assembly from a specimen of <i>Carex rostrata</i> (Bottle Sedge; Streptophyta; Magnoliopsida; Poales; Cyperaceae). The genome sequence has a total length of 382.30 megabases. Most of the assembly is scaffolded into 35 …

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Genome Assembly