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The role of plasma and serum proteomics in characterizing human disease, identifying biomarkers, and advancing diagnostic technologies is rapidly increasing. However, there is an ongoing need to improve proteomic workflows in terms of accuracy, reproducibility, …
Read PDFCirculating blood proteomics enables minimally invasive biomarker discovery. Nanoparticle-based circulating plasma proteomics studies have reported varying number of proteins (ca 2000-7000), but it remains unclear whether a higher protein number is more informative. Here, we …
Read PDF<b>Background</b>: Zangqing '1127', a hull-less barley type recognized for its high β-glucan content, holds significant agricultural and nutritional potential. Nonetheless, the molecular mechanisms underlying the degradation of β-glucan during barley germination have yet to be …
Read PDFIntestinal mucin glycan-degrading bacteria are important for mucus turnover, stimulating mucus production, and producing beneficial metabolites. The mucin-degrading bacteria require various enzymes to break down mucin O-glycans. In this study, mucin glycan-degrading bacteria Akkermansia muciniphila, …
Read PDF<h4>Background/aims</h4>Hepatocellular carcinoma (HCC) is one of the leading causes of cancer-related mortality worldwide and is driven by metabolic reprogramming that supports tumor growth and progression. A common missense genetic variant (rs2642438, p.A165T) in mitochondrial amidoxime …
Read PDFBottom-up proteomics is a powerful technique for comprehensive analysis of proteins by proteolytic cleavage followed by LC-MS/MS to identify the resulting peptides. Trypsin is the gold-standard protease for bottom-up proteomics, though its cleavage specificity limits …
Read PDF<h4>Background</h4>Aflatoxin B<sub>1</sub> (AFB<sub>1</sub>), a potent mycotoxin, commonly contaminates feeds like maize and soybean, jeopardizing animal reproduction. Although AFB<sub>1</sub> exposure is known to cause oxidative stress, immune activation, and cell death in oocytes and early embryos …
Read PDFCapillary zone electrophoresis (CZE)-mass spectrometry (MS) has attracted tremendous attention in top-down proteomics (TDP). However, its reproducibility and long-term repeatability for TDP remain concerns, most likely due to capillary coating. Here, we present an improved …
Read PDFBACKGROUND: The current phenotyping of chronic rhinosinusitis with nasal polyps (CRSwNP) into eosinophilic (eCRSwNP) and non-eosinophilic (non-eCRSwNP) subtypes is increasingly insufficient to address complex clinical challenges, especially as some non-eCRSwNP patients have poor prognoses. Identifying …
Read PDFMany diseases, including diabetes and hypertension, can lead to kidney damage, often resulting in long-term health complications and organ failure. Both animal and human studies have documented a causal relationship between high dietary sodium intake …
Read PDFLon protease is a global regulator in bacteria affecting biofilm formation, antibiotic resistance, toxin-antitoxin complexes, and proteolysis of damaged proteins. In <i>Klebsiella pneumoniae</i>, Lon has roles in iron response, protein transport, and membrane assembly. Here, …
Read PDF<b>Background</b>: Timely screening and diagnosis are critical for improving prognosis and survival in gastric cancer. Building on prior serum proteomics findings, this study evaluated two novel early-stage diagnostic biomarkers, CFL1 and TAGLN2, using retrospective and …
Read PDFFeline calicivirus (FCV) is a highly variable RNA virus that infects domestic cats and circulates endemically within feline populations, causing a wide spectrum of clinical manifestations, from asymptomatic infections to severe disease. Genomic analysis of …
Read PDFTranslational errors (TEs) result in a mismatch between mRNA codons and the amino acids (AAs) of the corresponding protein. Unlike DNA mutations or RNA editing, where nucleotide sequences can be used to infer AA substitutions, …
Read PDFBACKGROUND: Plasma and cerebrospinal fluid are complementary sources of biomarkers for neurodegenerative diseases. The wide dynamic range of protein abundances, particularly in plasma, hampers detection of low-abundance proteins. Depletion of high-abundance proteins and efficient enzymatic …
Read PDFThe Epstein-Barr virus (EBV) is a human gamma-herpesvirus which infects over 90% of the global population and is associated with lymphoid and epithelioid cancers. After infection, EBV enters a latent state in B cells, whereby …
Read PDFImmunotherapy offers promising opportunities to improve immune-mediated control of chronic myeloid leukemia (CML), but its success depends on identifying antigens uniquely associated with CML. Protein kinases regulate signaling and protein turnover, influencing which peptides are …
Read PDFTo assess the potential for high resolution ion mobility (HRIM) as an alternative means of precursor isolation for mass spectrometry fragmentation analysis we performed a meta-analysis of predicted tryptic peptide features from the human proteome …
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