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The Bonin flying fox (Pteropus pselaphon) is an endemic species of the Ogasawara Islands, Japan, and is classified as Critically Endangered. Here, we report a genome assembly of P. pselaphon, constructed using Nanopore long reads …
Read PDFWe present the complete genome sequence of <i>Erwinia spp. str</i>. LJJL01, isolated from waste charcoal in Colorado, USA, using Oxford Nanopore sequencing. This sequence provides important insights into this bacterium's metabolic and catabolic robustness to …
Read PDFEmergomyces (Ajellomycetaceae, Onygenales) is a genus of dimorphic fungal pathogens that cause severe, opportunistic infections around the world. We used long-read nanopore sequencing to generate de novo genome assemblies followed by comparative analyses for the …
Read PDFWe present a high-quality, chromosome-scale genome assembly for the redlip blenny Ophioblennius macclurei (family Blenniidae). The assembly was generated using a combination of Oxford Nanopore long-read sequencing, Illumina short-read data, and Hi-C scaffolding technology. The …
Read PDFChikungunya virus (CHIKV) poses an increasing global public health threat, as evidenced by the significant 2025 Foshan outbreak in China. Rapid, whole-genome sequencing (WGS) is critical for outbreak response but is challenged by primer mismatches …
Read PDFIn agriculture, biosecurity, and human health, the rapid and accurate detection of pathogens and pests is crucial. Our study investigates the sensitivity and practicality of six guide RNA (gRNA) production methods for use in Nanopore …
Read PDFWe report the complete genome of calcite-precipitating <i>Cytobacillus kochii</i> strain ITBMC_116, isolated from limestone karst in Vietnam. Hybrid Illumina-Nanopore sequencing yielded a 4.83-Mbp chromosome and an 87.4-kbp plasmid. This resource supports studies of microbially induced …
Read PDFLong-read RNA sequencing (RNA-seq) technologies have revolutionized transcriptomic research by enabling the sequencing of full-length RNA molecules, thus providing a more accurate characterization of complex transcript isoforms than traditional short-read approaches. In this study, we …
Read PDF<h4>Objectives</h4>The fruiting body of Rigidoporus glomeratus is a traditional medicinal herb employed by indigenous ethnic communities in the Gaoligong Mountain region. This fungus is known to be rich in various metabolites, including polyphenols, polysaccharides, flavonoids, …
Read PDFMulti-Locus Sequence Typing (MLST) is a key method for allocation of Sequence Types (STs) for bacterial isolates. Traditionally, this is performed by the Sanger sequencing method, which can be highly time-consuming and laborious. In this …
Read PDFNanopore sensors offer exceptional sensitivity for detecting single molecules, making them ideal for early disease diagnostics. In this study, we present a multiplexed nanopore-based assay that combines DNA-barcoded probes with advanced computational analysis to detect …
Read PDFSequencing of the 16S ribosomal RNA (rRNA) gene is an important tool in addition to conventional methods for the identification of bacterial pathogens in human infections. In polymicrobial samples, Sanger sequencing can produce uninterpretable chromatograms. …
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Read PDFMealybugs are aggressive pests worldwide, but few complete mitochondrial genomes have been reported. Here, we got the complete mitochondrial genome of the cotton mealybug, <i>Phenacoccus solenopsis</i> Tinsley, using Oxford Nanopore Technology. It is 14743 bp …
Read PDFThe complete genome sequence of <i>Kluyveromyces marxianus</i> strain MCHA was obtained using Illumina and Oxford Nanopore sequencing technologies. The genome comprises nine scaffolds, corresponding to the eight nuclear chromosomes and the mitochondrial genome.
Read PDFPathogenic GAA repeat expansions in FGF14 are an established cause of late-onset cerebellar ataxia, but have not been linked to Parkinson's disease. Given emerging evidence that repeat expansions in ataxia-associated genes like RFC1 can contribute …
Read PDF<i>Stenotrophomonas maltophilia</i> ESTM1D_MKCAZ16_6a (Sm18) is a multidrug-resistant strain isolated from river sediments in Morelos, Mexico. It is capable of replicating within acid Rab7A-positive <i>Acanthamoeba castellanii</i> phagosomes. The complete genome was assembled from Oxford Nanopore and …
Read PDF<i>Mycoplasma genitalium</i> is a fastidious human pathogen with increasing antimicrobial resistance, yet its genomic landscape remains poorly characterized due to difficulties with culture, including prolonged incubation periods and low DNA yields from both clinical and …
Read PDFThe first influenza A(H5N1) human case associated with the A(H5N1) dairy cattle outbreak in the United States was identified in April 2024. The U.S. CDC response to this outbreak was activated days later and remained …
Read PDF<h4>Objective</h4>Premature ovarian insufficiency (POI) with short stature and mild intellectual disability can have diverse genetic etiologies. We aimed to decipher the genetic basis of this complex phenotype in a 26-year-old female with a karyotype lacking …
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