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Wheat Snow Mold (WSM), caused by Microdochium species, poses a serious threat to global wheat production. Despite its importance, the genetic and molecular mechanisms of Microdochium remain poorly understood. In particular, genome-wide differences between M. …
Read PDF<b>Background/Objectives</b>: Short-read-sequencing (SRS) is currently the standard for genetic testing in inherited human diseases. Intrinsic limitations include PCR dependency, restricted read length, and challenges in identifying structural variants (SVs), copy number variations (CNVs), and intronic …
Read PDFDNA methylation has been proposed as an epigenetic driver of phenotypic plasticity in social insects, yet experimental evidence remains limited. Even less is known about the role of epigenetic mechanisms underlying behavioural and social polymorphism. …
Read PDFAlthough full-length 16S rRNA gene sequencing has substantially improved taxonomic resolution compared to short-read approaches, a high proportion of unclassified taxa are reported in rumen microbiome studies. This limitation is largely driven by platform-specific analytical …
Read PDFContinuous emergence of new severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants may influence viral transmission dynamics and alter interactions with the respiratory microbiota, potentially increasing the risks of reinfection. This study investigated cases of …
Read PDFShort-read assembly of the African swine fever virus (ASFV) genome is challenging due to the presence of inverted terminal repeat (ITR) and hairpin loop sequences, which often cause ambiguity in contig reconstruction. In this study, …
Read PDF<h4>Purpose</h4>Cell-free DNA (cfDNA) fragmentation patterns carry biological information beyond fragment length, revealing nuclease activity, chromatin organization, and tissue of origin. Fragmentomics has emerged as a powerful approach to improve circulating tumor DNA (ctDNA) detection, particularly …
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Read PDF<b>Background/Objectives</b>: BrdU (5'-bromo-2'-deoxyuridine), a synthetic thymidine (T) analog, is widely used to study cell proliferation and DNA synthesis. To precisely identify where and when DNA replication starts and terminates, it is essential to determine the …
Read PDF<i>Aedes albopictus</i> has recently established self-sustaining populations in Hungary, but its microbiota-which may influence vector competence-remains poorly understood. We used Oxford Nanopore long-read sequencing for full-length 16S rRNA gene profiling of adult <i>Ae. albopictus</i> from …
Read PDF<h4>Background</h4>Central nervous system (CNS) infections pose a significant public health challenge in resource-limited settings. Traditional culture-based and targeted molecular diagnostic methods have limitations in sensitivity and speed. This study retrospectively analyzed the data and cerebrospinal …
Read PDFIn March of 2025 the UK's first Marine Citizen Science Week recruited Citizen Scientists from around the country to participate in a series of activities exploring the ecology and biodiversity of rocky shorelines at sites …
Read PDFWe report the complete genome of <i>Burkholderia pseudomallei</i> strain 490f, isolated from a ceftazidime treatment failure case in Northeast Thailand. Oxford Nanopore and Illumina sequencing techniques revealed two circular chromosomes. A 150 kb region on …
Read PDFHypsibarbus vernayi (2n = 50), a medium-sized barb distributed in the Mekong River basin, is widely consumed in its native range and holds significant potential for commercial aquaculture development. To date, there is no high-quality …
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Read PDFAtmospheric pollution poses a major threat to human health, causing millions of deaths worldwide each year. Green infrastructures may help mitigate the toxicity of airborne contaminants through the combined action of plants and their associated …
Read PDFDNA methylation is a key epigenetic modification involved in various biological processes. However, its role in <i>Nosema ceranae</i> remains poorly understood. In this study, we employed Oxford Nanopore Technology sequencing to identify whole-genome DNA methylation …
Read PDF<b>Background/Objective</b>: Timely identification of bloodstream pathogens is critical for sepsis management; however, PCR inhibitors such as sodium polyanetholesulfonate (SPS) in blood culture broth compromise nucleic acid recovery and long read sequencing. We assessed whether coupling …
Read PDFRecent studies have revealed that the long-recognized link between the historically defined <i>Fusobacterium nucleatum</i> group and colorectal cancer is largely driven by <i>Fusobacterium animalis</i>. This species, along with two others (<i>Fusobacterium polymorphum</i> and <i>Fusobacterium vincentii</i>), …
Read PDF<h4>Background</h4>Long-read sequencing has revolutionised metagenomics through improved metagenome assembly, taxonomic classification and functional characterisation. Automation can enhance the throughput, reproducibility, and accuracy of library preparation. However, the validation of automated library preparation protocols remains undetermined …
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