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1474 results

Full text 2026

Emergence and genomic characterization of hypervirulent ST23/K1 <i>Klebsiella pneumoniae</i>: local epidemiology and global context

Bezdicek M, Jakubickova M, Bitusikova V, et al.

<h4>Introduction</h4>Hypervirulent <i>Klebsiella pneumoniae</i> (hvKp) of the K1/ST23 lineage is an emerging global threat associated with invasive community-acquired infections. Increasing reports of virulence-resistance convergence highlight the need for genomic surveillance, particularly within Europe where data remain …

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Nanopore Sequencing
Full text 2026

Genomic characterization of &lt;i&gt;mcr-1.1&lt;/i&gt;-positive &lt;i&gt;Salmonella&lt;/i&gt; Kentucky ST198 from a captive Asian houbara bustard reveals links to broiler poultry in the United Arab Emirates

Carrasco Muñoz M, Pérez de Vargas A, Alhebshi M, et al.

The Asian houbara bustard (<i>Chlamydotis macqueenii</i>) is a vulnerable species supported by conservation breeding programs internationally, as well as in the United Arab Emirates (UAE). Infectious diseases caused by antimicrobial-resistant pathogens represent an emerging threat …

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Nanopore Sequencing
Full text 2025

Resistome and Phylogenomics of &lt;i&gt;Escherichia coli&lt;/i&gt; Strains Obtained from Diverse Sources in Jimma, Ethiopia

Gashaw M, Gudina EK, Froeschl G, et al.

<b>Introduction</b>: In recent years, antimicrobial resistance (AMR) rates have increased significantly in bacterial pathogens, particularly extended beta-lactam resistance. This study aimed to investigate resistome and phylogenomics of <i>Escherichia coli</i> (<i>E. coli</i>) strains isolated from various …

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Nanopore Sequencing
Full text 2025

Implementation of a Multiplex PCR Amplification System Combined With Next-Generation Genome Sequencing to Decipher the Circulation of Human Coronavirus 229E Lineages in Southern France

Hikmat H, Py J, Boschi C, et al.

Coronaviruses rapidly evolve and are prone to new virus emergence. Human coronavirus (HCoV)-229E is one of the seven coronaviruses (aside HCoV-OC43, HCoV-HKU1, HCoV-NL63, SARS-CoV, MERS-CoV, SARS-CoV-2) causing respiratory infections in humans. Genomic data are very …

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Nanopore Sequencing
Full text 2025

Characterisation of the genome and secretome of &lt;i&gt;Phytophthoracryptogea&lt;/i&gt; and &lt;i&gt;P.erythroseptica&lt;/i&gt;

Hirooka Y, Villanueva O, Ponomareva E, et al.

<i>Phytophthoracryptogea</i> Pethybr. & Laff. and <i>P.erythroseptica</i> Pethybr. are oomycetes that cause root rot diseases of multiple plant species, including serious diseases of potato. These two species of <i>Phytophthora</i> were originally reported in Ireland more than …

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Nanopore Sequencing
Full text 2026

Plasmid-borne &lt;i&gt;mcr-4.3&lt;/i&gt; in &lt;i&gt;Acinetobacter nosocomialis&lt;/i&gt; recovered from imported beef cattle: Genomic characterization and one health implications

Xu H, Li Y, Hu S, et al.

<h4>Background</h4>The emergence of mobile colistin resistance determinants in clinically relevant Acinetobacter species poses a growing challenge to antimicrobial stewardship and One Health surveillance. Although <i>mcr-4.3</i> has been reported in <i>Acinetobacter</i>, its occurrence in imported food …

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Bioinformatics Nanopore Sequencing
Full text 2025

Hybrid &lt;i&gt;de novo&lt;/i&gt; genome assembly data and comparative genomics of &lt;i&gt;Fusarium chlamydosporum&lt;/i&gt; isolated from infected blackberry fields

Pashkov A, Martínez-Hernández JP, Herrera-Estrella A, et al.

Here, we present a dataset of ITS and β-tubulin sequences, along with a phylogenetic analysis using these molecular markers, for six putative <i>Fusarium</i> strains isolated from blackberry field soil. Additionally, we report the sequencing and …

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Nanopore Sequencing
Full text 2022

Simultaneous measurement of the size and methylation of chromosome 4qA-D4Z4 repeats in facioscapulohumeral muscular dystrophy by long-read sequencing

Hiramuki Y, Kure Y, Saito Y, et al.

<h4>Background</h4>Facioscapulohumeral muscular dystrophy (FSHD) is an autosomal dominant muscular disorder characterized by asymmetric muscle wasting and weakness. FSHD can be subdivided into two types: FSHD1, caused by contraction of the D4Z4 repeat on chromosome 4q35, …

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Nanopore Sequencing
Full text 2022

Comparative Analysis of Short- and Long-Read Sequencing of Vancomycin-Resistant Enterococci for Application to Molecular Epidemiology

Oh S, Nam SK, Chang HE, et al.

Vancomycin-resistant enterococci (VRE) are nosocomial pathogens with genetic plasticity and widespread antimicrobial resistance (AMR). To prevent the spread of VRE in the hospital setting, molecular epidemiological approaches such as pulsed-field gel electrophoresis and multilocus sequence …

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Nanopore Sequencing
Full text 2024

Long-Read Sequencing and <i>De Novo</i> Genome Assembly Pipeline of Two <i>Plasmodium falciparum</i> Clones (<i>Pf</i>3D7, <i>Pf</i>W2) Using Only the PromethION Sequencer from Oxford Nanopore Technologies without Whole-Genome Amplification

Delandre O, Lamer O, Loreau JM, et al.

Antimalarial drug resistance has become a real public health problem despite WHO measures. New sequencing technologies make it possible to investigate genomic variations associated with resistant phenotypes at the genome-wide scale. Based on the use …

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Nanopore Sequencing