Hybrid <i>de novo</i> genome assembly data and comparative genomics of <i>Fusarium chlamydosporum</i> isolated from infected blackberry fields

Here, we present a dataset of ITS and β-tubulin sequences, along with a phylogenetic analysis using these molecular markers, for six putative <i>Fusarium</i> strains isolated from blackberry field soil. Additionally, we report the sequencing and hybrid assembly of one of these strains, identified as <i>Fusarium chlamydosporum</i>. Whole-genome sequencing of this strain was conducted using Illumina NovaSeq 6000 and Oxford Nanopore MinION technologies, resulting in a high-quality hybrid assembly of approximately 37 Mb across 23 contigs, with over 99 % completeness. Phylogenomic analysis, incorporating 88 <i>Fusarium</i> genomes, confidently placed this strain within the <i>F. chlamydosporum</i> clade. This assembly is now the NCBI reference genome for <i>F. chlamydosporum</i>, providing a valuable resource for future genomic and evolutionary studies of this species.