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Wetland ecosystems provide interfaces for the transmission of microbial pathogens and antimicrobial resistances (AMR) between migratory birds, wild and domestic animals, and human populations. The efficient surveillance of wetlands is, however, challenging, since the typically …
Read PDFShort nasal swabs (SNS) have potential advantages of lower costs, collection time and training of personnel than deep nasopharyngeal swabs (DNPS) for detecting bovine respiratory disease (BRD) pathogens. This study examined differences between DNPS and …
Read PDFDNA-based methods and developments of sequencing technologies are integral to macrobenthos biodiversity studies, and their implementation as standardized monitoring methods is approaching. Evaluating the efficacy and reliability of these technological developments is crucial for macrobenthos …
Read PDFThe emergence of nanopore sequencing technology has the potential to transform metagenomics by offering low-cost, portable, and long-read sequencing capabilities. Furthermore, these platforms enable real-time data generation, which could significantly reduce the time from sample …
Read PDFLow primary productivity in Barents Sea surface waters and limited nutrient flux to the seafloor favor nitrification and nitrogen fixation in deep waters, resulting in a dearth of organic substrates in local sediments. The addition …
Read PDFBegonia fimbristipula Hance (Begoniaceae) is a valuable medicinal herb that is classified as a protected species in Guangdong Province, China. In this study, we present a chromosome-level genome assembly of B. fimbristipula, aiming to facilitate …
Read PDFWe report the whole-genome sequence of <i>Diaporthe australafricana</i> Crous & J.M. van Niekerkusing using Oxford Nanopore long-read sequencing and Illumina short-read sequencing. The hybrid genome consists of 11 contigs with a total length of 53.509 …
Read PDFSpliced leader (SL) trans-splicing occurs in a wide range of eukaryotes and plays a critical role in processing mRNAs derived from operon structures. However, current research on this mechanism remains limited, partly due to the …
Read PDF<i>Scedosporium</i> species are filamentous fungi with inherent broad antifungal resistance that pose opportunistic infection threats. We present draft genome assemblies of <i>S. aurantiacum</i> (11 contigs) and <i>S. apiospermum</i> (9 contigs), derived from Oxford Nanopore sequencing …
Read PDFThe COVID-19 pandemic significantly accelerated the development of genomic surveillance capabilities worldwide, though equitable access remains a challenge. On 12 March 2020, Guinea, a low-income country in West Africa, reported its first COVID-19 case; however, …
Read PDFRabies remains a major public health concern in Thailand. Nucleoprotein (N) and glycoprotein (G) genes from eight rabies-positive specimens collected during 2022 to 2024 were sequenced using Oxford Nanopore. Assemblies and phylogenetic analyses indicate genetic …
Read PDF<h4>Introduction</h4>Malaria remains a major public health challenge across sub-Saharan Africa, with Plasmodium falciparum responsible for the vast majority of cases and deaths. In Rwanda, although control measures have led to significant progress, malaria continues to …
Read PDFRibosomal RNAs (rRNAs) serve as species-defining markers and undergo processing steps, such as excision of intervening sequences (IVSs). Direct analysis of native rRNAs is hampered by enzyme-induced biases and by the high conservation of rRNA …
Read PDFDNA methylation offers an objective method to assess the impact of smoking. In this work, we conduct a Bayesian EWAS of smoking pack years (n = 17,865, ~850k sites, Illumina EPIC array) and extend it …
Read PDFMolar incisor hypomineralization (MIH) is a dental condition that affects the enamel of permanent molars and/or incisors, often leading to tooth decay. Although several etiological hypotheses have come forward, including prenatal medical problems and postnatal …
Read PDF<h4>Background</h4>Carbapenem-resistant Enterobacterales pose a significant public health threat, particularly when detected in food-producing animals and retail meat. Although carbapenems are not used in European Union animal production, sporadic cases of carbapenemase-producing <i>Escherichia coli</i> have emerged …
Read PDFThe ongoing development of human artificial chromosomes (HACs) will permit investigation into essential centromere processes and the means to deliver large genetic cargoes to target cells. Starting with large (~750 kb) yeast artificial chromosome (YAC)-based …
Read PDFAfrican swine fever virus (ASFV) is highly transmissible and can cause up to 100% mortality in pigs. The virus has spread across most regions of Asia and Europe, resulting in the deaths of millions of …
Read PDFTwo yeast strains, PYCC 10015 and PYCC 10016, were isolated from soil from an Irish forest. Sequence analysis of the internal transcribed spacer (ITS) region (ITS1-5.8S-ITS2) of the rRNA gene repeat, and the D1/D2 domain …
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