Full text 2025

The chromosomal genome sequence of the stone sponge <i>Petrosia ficiformis</i> (Poiret, 1789) and its associated microbial metagenome sequences

Steindler L, Maldonado M, Pita L, et al.

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Abstract

We present a genome assembly from an individual <i>Petrosia ficiformis</i> (stone sponge; Porifera; Demospongiae; Haplosclerida; Petrosiidae). The genome sequence is 191.3 megabases in span. Most of the assembly is scaffolded into 18 chromosomal pseudomolecules. The mitochondrial genome has also been assembled and is 18.89 kilobases in length. Gene annotation of the host organism assembly identified 18,339 protein coding genes. The metagenome of the specimen was also assembled, and 112 binned bacterial genomes were identified, including 57 high-quality MAGs. Besides MAGs characteristic of HMA sponge symbionts (i.e., Chloroflexota, Acidobacteriota), the <i>P. ficiformis</i> specific symbiont <i>Candidatus</i> Synechococcus feldmanni (formerly <i>Aphanocapsa feldmanni</i> (Cyanobacteriota) was recovered, as well as notably MAGs of several candidate phyla ( <i>Candidatus</i> Latescibacteria, Poribacteria, Tectomicrobia, Dadabacteria, Kapabacteria and Binatia).

Keywords

Chromosomal Genome sequence Petrosia Ficiformis Haplosclerida Stone Sponge