Telomere-to-telomere genome assembly of the Fraxinus mandshurica Rupr

Fraxinus mandshurica Rupr, an important timber species, is widely distributed throughout northeastern China. In our study, we assembled the telomere-to-telomere (T2T) genome of F. mandshurica, generated by using Illumina short-read, PacBio HiFi long-read and Hi-C sequencing. The genome size was 781.40 Mb and the scaffold N50 length was 34.29 Mb. 39 contigs were reliably scaffolded into 23 T2T chromosomes. More than 98.70% of the BUSCO genes from the embryophyta_odb12 dataset were successfully hit in the genome. We found that 61.01% of the sequences were repetitive, and 35,009 protein-coding genes were annotated, more than 99.22% were successfully functionally detected. Overall, this T2T-level genome assembly provides a valuable genomic platform that will facilitate studies of evolutionary biology, functional genomics, and molecular breeding in F. mandshurica.