Single-cell RNA-seq data of wild type and fli1b mutant zebrafish embryos

Fli1b is an ETS transcription factor, which has been previously implicated in zebrafish vascular and hematopoietic development. Here we present single cell RNA sequencing data from wild-type and maternal zygotic <i>fli1b</i> mutant zebrafish embryos at 24 h post fertilization. Single-cell suspensions were obtained from approximately 40 whole maternal-zygotic (MZ) <i>fli1b</i> mutant and sibling parent wild-type embryos and subjected to RNA sequencing using the 10X Genomics Chromium platform. Following bioinformatic analysis, 34 distinct cell clusters were identified in the integrated wild-type and <i>fli1b</i> mutant dataset. The clusters were subsequently annotated based on expression of marker genes. These data will be valuable for further studies of the molecular mechanisms involved in vascular and hematopoietic development. In addition, the obtained transcriptomes of multiple cell types will be useful to investigate other developmental mechanisms in zebrafish and other models.