Outbreak of dengue fever in Ghana: The emergence of DENV-1 serotype

<h4>Background</h4>Dengue, a mosquito-borne virus continues to be a public health concern in the tropics and subtropical parts of Africa. Due to the increase of urbanization, climate change and trans-Atlantic trade, the transmitting vector, Aedes aegypti, has become prevalent hence the rapid growth of the disease, globally. In Ghana, there have been sporadic laboratory-confirmed cases of dengue reported over the years through surveillance activities. However, despite the detection of these cases, Ghana had never experienced a major outbreak (unlike its neighboring countries) until July 2024.<h4>Methods</h4>During this outbreak, a total of 1471 suspected dengue fever specimen received from various health facilities in Ghana in NMIMR for molecular diagnostic testing using a RT-qPCR assay for dengue, chikungunya and Zika viruses and selected positives sequenced using Illumina Next Generation Sequencing.<h4>Results</h4>Dengue fever virus RNA was detected from 206 samples and serotyped as DENV-1 with one DENV-3 coinfection. Thirty-nine genomes were successfully generated after sequencing and phylogenetic analysis of DENV-1 strains revealed two main clusters with AFI isolates in Ghana and isolates from other West African countries (Côte d'Ivoire, Burkina Faso, Benin, and Senegal) circulating between 2017-2019. In 2023, DENV-1 was frequently isolated which could account for it being the predominant serotype transmitted in the recent outbreak.<h4>Conclusion</h4>The outbreak response and the case management procedures deployed by the health authorities during this outbreak were swift and was enough to prevent a fatal difficult-to-control situation. With the absence of a widely accepted commercialized vaccine and treatment for dengue fever, there is a need to enhance surveillance activities and control the vectors which can transmit DENV in-country to curb the occurrence of outbreaks.