Mycobacteria Amplicon Sequencing Tool: automated resistance prediction and lineage classification for <i>Mycobacterium tuberculosis</i>
Abstract
The Mycobacteria Amplicon Sequencing Tool (https://github.com/guthrielab/MAST) is a modular Nextflow pipeline for antimicrobial resistance prediction and lineage classification of <i>Mycobacterium tuberculosis</i> from amplicon or whole-genome sequencing data sets. The workflow automates read processing, variant calling, and annotation to produce standardized, human-readable reports in DOCX and TSV format.