<i>Acinetobacter uliginosus</i> sp. nov. and <i>Acinetobacter halobius</i> sp. nov. isolated from soil
Abstract
Two bacterial strains, designated BSP-53<sup>T</sup> and BSP-153<sup>T</sup>, were isolated from soil samples collected from Beijing Olympic Forest Park, China. Phylogenomic reconstruction based on core genes robustly positioned the strains as two distinct evolutionary lineages within the genus <i>Acinetobacter</i>. Both strains were Gram-negative, aerobic, non-motile and short rods. Their major cellular fatty acids consisted of summed feature 3 (C<sub>16 : 1</sub> <i> ω</i>7<i>c</i>/C<sub>16 : 1</sub> <i> ω</i>6<i>c</i>), C<sub>18 : 1</sub> <i> ω</i>9<i>c</i>, C<sub>16 : 0</sub>, C<sub>12 : 0</sub> and C<sub>12 : 0</sub> 3-OH. The predominant polar lipids included diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The average nucleotide identity values between the novel strains and their closest <i>Acinetobacter</i> type strains were below 95%, with corresponding digital DNA-DNA hybridization values below 70%. Based on polyphasic taxonomic characterization, including chemotaxonomic, phenotypic, phylogenetic and genomic analysis, we propose that strains BSP-53<sup>T</sup> and BSP-153<sup>T</sup> represent two novel species of the genus <i>Acinetobacter</i>, for which the names <i>Acinetobacter uliginosus</i> sp. nov. and <i>Acinetobacter halobius</i> sp. nov. are designated. The type strains are BSP-53<sup>T</sup> (=CGMCC 1.62070<sup>T</sup>=KCTC 8190<sup>T</sup>) and BSP-153<sup>T</sup> (=CGMCC 1.62071<sup>T</sup>=KCTC 8191<sup>T</sup>), respectively.