Full text 2023

Long-read Sequencing and de novo Genome Assembly of Three Aspergillus fumigatus Genomes

Hemmings SJ, Rhodes JL, Fisher MC.

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Abstract

Aspergillus fumigatus is a genetically diverse fungal species, which is near ubiquitous in its global distribution and is the major cause of the life-threatening disease invasive aspergillosis. We present 3 de novo genome assemblies that were selected to be representative of the genetic diversity of clinical and environmental A. fumigatus. Sequencing using long-read Oxford Nanopore and subsequent assembly of the genomes yielded 10-23 contigs with an N50 of 4.05 Mbp to 4.93 Mbp.

Keywords

Aspergillus fumigatus Azole Resistance De Novo Genome Assembly Fungal Genome Oxford Nanopore Sequencing