Interspecies rice versus Arabidopsis thaliana protein-protein interactome profiling by touch-down overlapping PCR coupled with HiFi long-read sequencing

Touch-down overlapping PCR coupled with HiFi long-read sequencing, a high-throughput method for large-scale profiling of protein-protein interactions based on stitch-PCR identified 7,726 high-confidence interactions between rice and Arabidopsis proteins by integrating a library-vs-library yeast two-hybrid strategy with optimized PCR and long-read sequencing.