Full text 2023

<i>De-novo</i> whole genome assembly of the orange jewelweed, <i>Impatiens capensis</i> Meerb. (Balsaminaceae) using nanopore long-read sequencing

Gadagkar SR, Baeza JA, Buss K, et al.

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Abstract

The plant family Balsaminaceae comprises only two genera, and they are a study in contrasts. While <i>Impatiens</i> is an impressively prolific genus, with over 1,000 species and more being discovered each year, its sister genus, <i>Hydrocera</i>, has one solitary species, <i>H. triflora</i>. The two genera also differ in geographic distribution and habitat type (<i>Impatiens</i> species are widely distributed in much of the Old World and N. America, while <i>H. triflora</i> is confined to wetlands specific to S. India, Sri Lanka, and SE Asia). Other contrasting features include plant habit, habitat, floral architecture, mode of seed dispersal, and a host of other traits. The family Balsaminaceae is therefore an excellent model for studying speciation and character evolution as well as understanding the proximal and evolutionary forces that have driven the two genera to adopt such contrasting evolutionary paths. Various species of the <i>Impatiens</i> genus are also commercially important in the ornamental flower industry and as sources of phytochemicals that are of medicinal and other commercial value. As a preliminary step towards studying the genomic basis of the contrasting features of the two genera, we have sequenced and assembled, <i>de novo</i>, the genome of an iconic <i>Impatiens</i> species from N. America, namely <i>I. capensis</i>, and report our findings here.

Keywords

De Novo Whole Genome Sequencing Impatiens Capensis Oxford Nanopore Technology Long Read Sequencing Orange Jewelweed