Genomic Features of the Micropredator <i>Lysobacter</i> sp. Hz25 Isolated from the Rhizosphere of <i>Hedysarum zundukii</i>

<i>Lysobacter antibioticus</i> Hz25 is a novel strain that was isolated from the rhizosphere of the relict endemic plant <i>Hedysarum zundukii</i> Peschkova (Fabaceae), which grows on carbonate soils in the Baikal region of Russia. This work presents the complete genome sequence of Hz25 (5.98 Mb, 66.94% GC), which was obtained using a hybrid assembly method combining Oxford Nanopore and Illumina sequencing. Phylogenetic analysis based on 47 <i>Lysobacter</i> genomes and an average nucleotide identity (ANI) value of 96% confirmed its affiliation with <i>L. antibioticus</i>. A comparative pan-genome analysis with three closely related strains (13-6, 76, and ATCC 29479) identified 554 strain-specific genes. This significant genomic plasticity likely reflects adaptation to the sharply continental climate, high insolation, and low free iron content of the native soil. The genome encodes a comprehensive micropredator arsenal, including: seven chitinase genes (GH18 and GH19 families); bacteriolytic enzymes (Blp, L1, L4, Ami); a complete type III secretion system (T3SS) with predicted effectors; type IV pili (including the PilZ-PilB regulatory complex); and siderophore biosynthesis genes (lysochelin). The genome contains genes <i>ars</i> of an arsenic resistance system, but lacks the ACR3 efflux pump, suggesting that these genes may have alternative functions. Genes involved in calcium homeostasis (Excalibur domain, Na⁺/Ca²⁺ antiporter) were also identified. These features make Hz25 a promising candidate for biocontrol applications in cold climates and metal-contaminated environments.