Full text 2026

Construction of saturated Tn-Seq libraries of <i>Brucella abortus</i> S19 for transposon insertion and effective density analysis across stress conditions

Knebel E, Sharma G, Curtis P, et al.

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Abstract

<i>Brucella abortus</i> is a globally significant zoonotic pathogen. To explore the functional relevance of its genes in acidic, oxidative, and antimicrobial stress conditions, we constructed high-density transposon sequencing libraries in the attenuated <i>B. abortus</i> S19 strain. Here, we present the results and analysis of transposon insertion frequencies and effective densities.

Keywords

Brucella Stress responses Tn-seq