Commentary: a review of technical considerations for planning an RNA-Sequencing experiment

There are a bewildering number of variables to consider when planning an RNA sequencing study (RNA-Seq). Here we discuss some of the considerations for an investigator initiating such studies, with a focus on library preparation, depletion techniques, considerations for data analysis, and finally, we consider the cost of such a study. The goal of this summary is to make the researcher aware of such considerations to be able to plan an analysis strategy prior to initiating an RNA-Seq study.