Chromosome-level genome assembly of predatory Eocanthecona furcellata
Abstract
Eocanthecona furcellata (Wolff, 1811) (Hemiptera: Pentatomidae: Asopinae), a predatory stinkbug, preys on diverse notorious insect pests, positioning it as a critical biocontrol agent in agricultural and forestry ecosystems with substantial regional application and promotion potential. We present a chromosome-level genome assembly of E. furcellata using PacBio HiFi long-read sequencing and Hi-C scaffolding technologies. The final genome spans 1.03 Gb, with a contig N50 of 2.35 Mb and a scaffold N50 of 135.69 Mb, achieving 97.0% BUSCO completeness with the Hemiptera_odb10 lineage dataset. Hi-C data resolved the assembly into 7 pseudochromosomes, representing the full karyotype. Repeat elements constitute 52.07% of the genome (approximately 520.07 Mb). We annotated 15,802 protein-coding genes, of which 90.72% were informatively assigned. This high-quality genome provides a foundational resource for advancing research on predatory bug biology, including genetic adaptations for digestion, molecular mechanisms of prey detection, and biocontrol optimization through functional genomics.