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<b>Background</b>: Untargeted proteomics enables quantitative host cell protein (HCP) determination in biotherapeutics, yet no workflow has been validated under ICH Q2(R2) for regulated quality control. <b>Methods</b>: A prospective total-error (TE) validation of label-free ddaPASEF proteomics …
Read PDFCartilage is characterized by a highly specialized extracellular matrix (ECM) secreted by chondrocytes and limited self-regenerative capacity. In vivo investigations of chondrogenesis are limited by difficult and traumatic access, especially in humans. While it is …
Read PDFProteomic and phenotypic cell sensitivity datasets are increasingly important for understanding chemoproteomics and the underlying drug mechanisms of action. Yet, integrating such heterogeneous datasets remains challenging due to inconsistent annotations, incompatible IDs, and variable data …
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Read PDFFood-induced anaphylaxis (FIA) is a life-threatening allergic reaction, while wheat-dependent exercise-induced anaphylaxis (WDEIA) is triggered by wheat ingestion plus cofactors. To elucidate their differences, we profiled serum extracellular vesicle (EV) proteomes from 240 participants, including …
Read PDFDemineralized bone matrices (DBMs) are widely used in bone replacement therapy. Bone tissue of either cancellous or cortical origin is decellularized, demineralized, and sterilized during processing, while retaining portions of native organic extracellular matrix (ECM) …
Read PDFMyosin-19 (Myo19) plays a crucial role in mitochondrial dynamics, cristae organization and ER-mitochondria contact sites. It regulates cytokinesis and inheritance of mitochondria to daughter cells. To better understand the dynamic molecular network of Myo19 during …
Read PDFBreast cancer remains a leading cause of cancer-related mortality worldwide, with obesity markedly increasing the risk in affected individuals. Liquid biopsy-based extracellular vesicles (EVs) offer a minimally invasive platform for molecular profiling of tumor-derived markers. …
Read PDF<h4>Background</h4>Endothelial cells express numerous microproteins (miPs) encoded by small open reading frames (smORFs), yet the biological function of most remains unknown. This study set out to characterize a novel 69 amino acid miP encoded within …
Read PDFOver the past two decades, proteomics has evolved from a descriptive cataloging of proteins to a sophisticated analytical science that provides deep insights into the dynamic complexity of biological systems [...].
Read PDFAutoinflammation of unknown origin remains amongst the most enigmatic of systemic autoinflammatory disorders (SAID), with systemic autoinflammatory symptoms in the absence of a molecular or clinical diagnosis with a recognized SAID. Here, we aim to …
Read PDF<h4>Purpose</h4>To understand the pathogenesis of the main lacrimal gland dacryops (ductular cyst) using proteomics and cellular profiling.<h4>Methods</h4>Four patients (mean age, 42.2 ± 18.4 years) with dacryops underwent cyst excision following the aspiration of the cyst …
Read PDFAstrocytes are increasingly implicated in the pathophysiology of schizophrenia (SCZ), yet how astrocytic dysfunction contributes to disease-relevant neuronal abnormalities remains unclear. Here, we used mass spectrometry-based proteomics to profile lysates (proteome) and secreted proteins (secretome) …
Read PDFMitochondrial dysfunction is a prominent hallmark of aging contributing to the decline of metabolic plasticity in late life. While genetic distortions of mitochondrial integrity elicit premature aging, the mechanisms leading to "natural" aging of mitochondria …
Read PDFDynamic metabolic engineering enables temporal redirection of microbial metabolism from biomass production to product synthesis. Here, we show that optogenetic control of protein kinase A (PKA) activity via light-regulated modulation of intracellular cyclic AMP (cAMP) …
Read PDF<h4>Background</h4>Parkinson's disease (PD) is the second most common neurodegenerative disorder with cognitive impairment as a prime non-motor symptom. Biomarkers can assist in detecting cognitive impairment, which normally follows movement dysfunction, and also assist in the …
Read PDFAbsence of the endosomal SNAREs vti1a and vti1b results in perinatal death and severe neuronal phenotypes in mice, while lack of one of these proteins results in minor phenotypes. Proteomic differences were investigated to obtain …
Read PDF<h4>Introduction</h4>Previous studies have examined the prediction of insulin resistance and type 2 diabetes (T2D) using plasma or saliva omics, but none have combined metabolomics and proteomics from multiple biofluids, such as plasma and saliva. Among …
Read PDFOxysterol-binding protein-like 2 (OSBPL2) is a lipid transfer protein that modulates intracellular lipid homeostasis; however, its regulatory role in coordinating iron and fatty acid homeostasis remains largely elusive. A recent study has reported a strong …
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