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The reality of an aging population demands a deeper understanding of aging as a biological process, rather than as a chronological descriptor. Chronological age poorly captures interindividual heterogeneity in physiological and functional decline, disease susceptibility, …
Read PDFThe embryo establishes a body plan and primes itself for organogenesis during gastrulation. As gastrulation is challenging to study in vivo, stem-cell-derived 'gastruloids' have emerged as powerful surrogates. Although transcriptomics and imaging have been applied …
Read PDF<h4>Background</h4>Adenovirus 26 (Ad26) vector-based vaccines have shown immunogenicity and safety in various infectious disease settings. However, the impact of each vaccine component (vector/insert) on the immune response has yet to be explored in a controlled …
Read PDFRheumatoid arthritis (RA) is a chronic autoimmune disorder characterized by persistent synovitis, in which fibroblast-like synoviocytes (FLSs) serve as the primary effector cells that drive the destruction of joints. Baicalin has previously demonstrated efficacy in …
Read PDFImmunomodulatory imide drugs (IMiDs) like lenalidomide and pomalidomide are effective in treating multiple myeloma (MM) but pose hematotoxicity risks by degrading neosubstrates Ikaros (IKZF1) and Aiolos (IKZF3). When these IMiD scaffolds are integrated into proteolysis …
Read PDF<h4>Background</h4>Tauopathies are neurodegenerative diseases all characterized by tau lesions in the brain. Nevertheless, a clinical and pathophysiological heterogeneity is present among them. This includes the dominant tau isoform found within aggregates (3R and/or 4R tau) …
Read PDFHigh-throughput proteomics requires efficient and highly reproducible sample processing, yet workflows─particularly for PTM profiling─remain complex and costly to fully automate. Here, we present a practical intermediate solution using manually operated 96-channel devices: the Gilson Platemaster …
Read PDFChlorogenic acid (CGA), which is ubiquitous in diverse botanical sources, demonstrates considerable anticancer potential through modulation of multiple targets or signaling pathways, thereby posing substantial challenges for mechanistic elucidation and target identification. Based on the …
Read PDFEscalating insecticide resistance in mosquito vectors threatens the durability of vector-borne disease control and increasingly constrains the effectiveness of core interventions. This resistance is a multilayered adaptive phenotype arising from the combined action of target-site …
Read PDFChronic kidney disease (CKD) is highly prevalent worldwide, yet structural kidney injury often develops long before changes in estimated glomerular filtration rate (eGFR) or persistent albuminuria are detected. Albuminuria is a well-established prognostic marker and …
Read PDFMissing values are a major challenge in the analysis of mass spectrometry proteomics data. Missing values hinder reproducibility, decrease statistical power for identifying differentially abundant proteins, and make it challenging to analyze low-abundance proteins. We …
Read PDFThe family of sulfur mustards, as potent alkylating agents, has been used as chemical warfare agents for over a century. The retrospective verification of exposure and, hence, the confirmation of alleged use of such agents, …
Read PDFSingle-cell proteomics (SCP) platforms are widely sought-after biomedical tools to complement existing omics technologies. Here, we present MiProChip, a microfluidic platform that integrates cell capture, lysis, protein digestion, tandem mass tag (TMT) labeling, on-chip pooling, …
Read PDF<h4>Background</h4>Hypertrophic cardiomyopathy (HCM) is the most common inherited heart disease, shows significant genetic and clinical heterogeneity, yet its molecular basis remains unclear. This study aims to uncover molecular insights behind HCM based on proteomic analysis.<h4>Methods</h4>We …
Read PDF<h4>Background</h4>Understanding synapse-specific effects of neuroinflammation can provide mechanistic and therapeutically relevant insights across the spectrum of neurological diseases.<h4>Methods</h4>We applied neuron-specific proteomic biotinylation <i>in vivo</i>, differential centrifugation of brain for crude synaptosome enrichment (P2 fraction) and …
Read PDFArginylation is a protein modification event in which cellular machinery recognizes a conserved N-terminal or side-chain motif and post-translationally installs an arginine residue to signal a protein for degradation. This modification affects protein function, stability, …
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