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We report the genome sequence of a human monkeypox virus isolated from an early Central European case. The 197,993 bp genome was assembled from amplicons sequenced using the Oxford Nanopore method. It has a GC …
Read PDFThe Western honeybee (Apis mellifera) is a crucial contributor to worldwide agriculture and ecological health but is experiencing wide population declines linked to Varroa mite infection. Formic Acid (FA) has been increasingly used to control …
Read PDF<h4>Main conclusion</h4>A haplotype-resolved, chromosome-scale AAC Mountainview sainfoin genome sequence along with organelle assemblies, methylome, and comparative tissue/stress-specific transcriptomes provide a solid foundation to support downstream sainfoin breeding efforts. Sainfoin (Onobrychis viciifolia Scop.) is an outcrossing, …
Read PDFCapybaras (Hydrochoerus hydrochaeris) play important ecological, cultural, and economic roles in Colombia, particularly in the Orinoco region. Their adaptability to human-modified environments increases contact with humans and animals, raising concerns about their role in zoonotic …
Read PDFIn recent years, Oxford Nanopore Technologies (ONT) has gained substantial attention across various domains of nucleic acid research, owing to its unique advantages over other sequencing platforms. Originally developed for long-read sequencing, ONT technology has …
Read PDFIn 2023, an estimated 10.8 million people developed tuberculosis, and 1.25 million people died from this disease, including 161,000 deaths in people with HIV (PWH) in whom tuberculosis remains the leading cause of death. Detecting …
Read PDFGenome sequences of <i>Helicobacter pylori</i> strains isolated from patients in Nariño, Colombia, with gastric lesions were assembled using Nanopore sequencing. Plasmids were detected in some strains and were predicted to be mobilizable, with relaxases of …
Read PDFZoonotic malaria, caused by parasites like Plasmodium simium, poses challenges to control and elimination efforts due to undetected reservoirs and altered transmission dynamics. Recent outbreaks in Brazil underscore the importance of P. simium spillovers from …
Read PDFThe genome of <i>Bacillus licheniformis</i> HB022, which was isolated from Daqu, a Chinese liquor starter, is 4,369,776 bp long with 4,501 protein-coding genes and a GC ratio of 46.2%. Keyword-based filtering from the functional annotations …
Read PDF<h4>Objective</h4>Malaria and dengue are two important infectious diseases in Indonesia, but information about their co-infections is still limited. This study aimed to describe malaria-dengue co-infections in Timika, Papua, including the clinical manifestations and genetic characteristics …
Read PDFChrysosplenium macrophyllum Oliv., a perennial herb native to China, is widely used in traditional medicine for its notable therapeutic properties. However, the absence of a reference genome has constrained its full potential for research and …
Read PDFAn exciting feature of nanopore sequencing is its ability to record multiomic information on the same sequenced DNA molecule. Well-trained models allow the detection of nucleotide-specific molecular signatures through changes in ionic current as DNA …
Read PDFThis report describes the complete genome sequence of the non-bioluminescent marine bacterium <i>Photobacterium piscicola</i> strain WVL24019, isolated from a one-year freshwater hatchery-raised rainbow trout in a non-recirculating system supplied by aquiferous water. The genome was …
Read PDFIn the past decade, third-generation sequencing technologies (such as PacBio (Pacific Biosciences) and Nanopore) have become gradually matured and are widely used for microbial taxonomy and quantification. Compared with Illumina sequencing, PacBio or Nanopore has …
Read PDFThe red swamp crayfish (<i>Procambarus clarkii</i>) is a globally important freshwater crustacean that exhibits pronounced sexual dimorphism, with males growing faster than females. However, the molecular mechanisms underlying sex differentiation in crustaceans remain poorly understood. …
Read PDFHow features characteristic of multicellular animals emerged in evolution is one of the key topics of modern evolutionary biology. We can get closer to answering that question by studying animals that occupy a sister taxon …
Read PDFNorovirus is a leading cause of acute gastroenteritis, with genogroup II (NoV-GII) being predominant. This study presents a novel genotyping system for norovirus GII, combining long-range PCR to amplify the complete ~3.2 kb RdRp-VP1 region …
Read PDF<h4>Introduction</h4>Antimicrobial resistance is an alarming public health problem, and comprehensive surveillance across environments is required to reduce its impact. Phenotypic testing and whole-genome sequencing of isolates are efficient, but culture-free approaches like metagenomic sequencing potentially …
Read PDFTranscriptomics is key to understanding how bacterial pathogens adapt and cause disease, but remains constrained by cost, technical, and biosafety issues, especially for highly virulent and/or regulated pathogens. Here, we present a streamlined and cost-effective …
Read PDFThis announcement describes the genome of <i>Paraburkholderia</i> sp. strain DD10, a bacterium originating from meadow soil that associates with hyphae of the arbuscular mycorrhizal fungus <i>Rhizophagus irregularis</i>. Oxford nanopore sequencing and genome assembly yielded a …
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