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Oncogenic driver mutations in non-small cell lung cancer (NSCLC) activate defined signaling pathways that sustain tumor growth and influence the immune landscape. Yet, how coordinated interactions among diverse cell populations within the tumor immune microenvironment …
Read PDFSingle-cell sequencing has revolutionized our understanding of cervical cancer (CC), revealing unprecedented cellular heterogeneity, tumor microenvironment (TME) dynamics, and molecular mechanisms underlying progression and therapy resistance. These technologies have identified distinct molecular subtypes (hypoxic, proliferative, …
Read PDFN6-methyladenosine (m<sup>6</sup>A) modifications are prevalent across all mammals and represent the most abundant type of epigenetic modification. With advancements in research, new methods for single-cell m<sup>6</sup>A modification sequencing and prediction have continuously emerged. These methods …
Read PDFCharacterizing the ancestry of donors in single-cell transcriptomic studies is crucial to ensure genetic homogeneity, reduce biases in analyses, identify ancestry-specific regulatory mechanisms and their downstream roles in disease, and ensure that existing datasets are …
Read PDFEpilepsy affects millions worldwide with many failing to respond to standard treatments. Drug-refractory epilepsy requiring surgical intervention often presents due to developmental aberrations, such as focal cortical dysplasia (FCD). In FCD type II, somatic mutations …
Read PDFEsophageal squamous cell carcinoma (ESCC) progression involves dynamic cellular state transitions and tumor microenvironment remodeling, accompanied by extensive transcriptional regulation reprogramming. Here, we systematically mapped the TF-mediated regulatory landscape underlying ESCC progression at single-cell resolution …
Read PDFFibrosis is an organ dysfunction caused by excessive deposition of fibrous components produced by parenchymal cells. Effective treatments are lacking for this progressive pathological condition that manifests in various organs and can lead to mortality. …
Read PDFThe integration of single-cell sequencing and organoid technologies has been transformative for biomedical research, enabling investigations of organ development, disease mechanisms, and therapeutic innovation at even finer resolutions. Organoids serve as 3D in vitro models …
Read PDFEndometriosis is characterized by progressive fibrosis and limited therapeutic options. Cuproptosis, a copper-dependent form of regulated cell death, has been implicated in multiple pathological conditions, but its relevance to fibroblast-mediated fibrotic progression in endometriosis remains …
Read PDF<h4>Background</h4>Giant juvenile fibroepithelial tumors of the breast are rare lesions that mainly affect adolescent girls. Their pathogenesis is poorly understood, and current management relies almost entirely on surgery, which may interfere with breast development. To …
Read PDF<h4>Background</h4>Immunometabolic remodeling is central to sepsis, yet robust glycolysis-associated biomarkers and their cell-type context remain unclear.<h4>Methods</h4>We integrated peripheral blood scRNA-seq (GSE175453) and a whole-blood microarray cohort (GSE100159). Glycolysis activity was scored by AUCell (HALLMARK_GLYCOLYSIS), hub …
Read PDF<h4>Background</h4>Alopecia areata (AA) is an autoimmune hair loss disorder. Although autoreactive CD8<sup>+</sup> T cells are central effectors, the roles of stromal and epithelial compartments in sustaining inflammation remain unclear.<h4>Methods</h4>This study analyzed publicly available scRNA-seq data …
Read PDFTuberculosis (TB) vaccine development is hindered by limited understanding of human immune responses to Mycobacterium tuberculosis. Using an aerosolized Mycobacterium bovis BCG human challenge model, we perform single-cell and bulk immune profiling of bronchoalveolar lavage …
Read PDFHere, we present a protocol to acquire, visualize, and quantify intracellular labile iron and oxygen signals at single-cell resolution using LiON (labile iron and oxygen notifier), a genetically encoded ratiometric reporter. We describe steps for …
Read PDF<h4>Background</h4>Guttate psoriasis (GP) is associated with streptococcal throat infection. Neutrophils are the first immune cells to respond to Group A Streptococcal (GAS) infection, but detailed analysis of their contribution to GP pathogenesis is lacking. The …
Read PDF<h4>Background</h4>Idiopathic pulmonary fibrosis (IPF) is a progressive fibrotic lung disease that has increasingly been associated with dysregulated mitochondrial quality control and dynamics. However, the molecular mechanisms underlying these alterations remain incompletely understood. This study aimed …
Read PDFMitochondria contain their own genome, mitochondrial DNA (mtDNA), which is under strict control by the cell nucleus. mtDNA occurs in many copies per cell and mutations often only affect a proportion of them, giving rise …
Read PDFThe tree shrew is a potential mammalian model for preclinical studies, but its immune system is not well understood. In this study, we utilized single-cell RNA sequencing (scRNA-seq) to construct a comprehensive cell atlas of …
Read PDFThe mechanisms that underlie protective immunity to Mycobacterium tuberculosis (Mtb) remain incompletely defined. To identify immune correlates associated with protection, we performed single-cell RNA sequencing of lung immune cells after aerosol Mtb infection of naïve …
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