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<h4>Aim</h4>Acute coronary syndrome (ACS) and ischemic stroke are major life-threatening conditions caused by atherosclerosis. Although the mechanisms of atherosclerosis appear to be broadly similar across different vascular beds, growing evidence suggests that there are morphological …
Read PDFThe immunosuppressive tumor microenvironment (TME) and intrinsic heterogeneity of ovarian cancer (OC) are primary drivers of therapeutic resistance and mortality. To deconvolute these complex dynamics and identify robust therapeutic targets, this study employed an integrative …
Read PDF<h4>Purpose</h4>Osteoporosis is a metabolic bone disorder characterized by reduced bone mass, impaired microarchitecture, and diminished bone strength, resulting in a significantly elevated risk of fractures. It is especially prevalent among older adults, particularly postmenopausal females, …
Read PDFThe gasdermin (GSDM) family mediates pyroptosis and membrane pore formation, but the causal, cell-type-specific roles of individual paralogues in immune-mediated inflammatory diseases (IMIDs) remain poorly defined. We combined single-cell cis-eQTL data from 14 peripheral blood …
Read PDFAcute myeloid leukemia (AML) is a molecularly archipelagic disease, characterized by profound cellular heterogeneity that fuels pathogenesis and therapy resistance. Traditional bulk sequencing approaches, by averaging signals across thousands of cells, have obscured the complex …
Read PDFThis review evaluates pulsed electric fields (PEF) as an emerging platform for single-cell bioprocessing in food applications. Connections are drawn between key mechanisms in electropermeabilization and applications, and a practical PEF process design framework is …
Read PDFThe endocrine system is crucial for maintaining overall homeostasis. However, its cellular signatures have not been elucidated during aging. Here, we conducted the first-ever single-cell transcriptomic profiles from eight endocrine organs in young and aged …
Read PDFWe introduce SCITO-seq2, an enhanced successor to SCITO-seq that integrates probe-based RNA detection with the established ultra-high-throughput protein profiling. SCITO-seq2 achieves robust quantification of transcripts and surface proteins across more than 100,000 cells, with a …
Read PDFCancer can recur when a subset of tumor cells, termed persister cells, survive therapy and re-enter the cell cycle. Through single-nucleus multi-omic profiling (single-nucleus RNA sequencing [snRNA-seq] and single-nucleus assay for transposase-accessible chromatin by sequencing …
Read PDFNumerous cell types relate to their immediate environment by exerting a three-dimensional pressure field on their environment, with components both longitudinal and transverse to the cell membrane. This pressure field can in principle be measured …
Read PDFMicrobial communities function as dynamic societies where intercellular communication governs collective behaviors. However, mapping these interaction networks has remained a fundamental challenge in microbiology. This study aims to decode the social networks of complex bacterial …
Read PDFDespite the therapeutic potential of engineered immune cell therapy against metastases, it faces challenges including cytokine-driven systemic toxicity, off-target biodistribution, and host rejection. Here, we develop red/far-red light-regulated individually encapsulated (RL/FRL-EnE) cells, integrating optogenetics with …
Read PDFHigh-throughput single-cell RNA sequencing (scRNA-seq) has substantially advanced plant transcriptional landscapes. However, decoding cell-type-specific transcriptional regulation in non-model crops like tomato (<i>Solanum lycopersicum</i>) remains challenging. An integrated computational pipeline was applied using high-dimensional weighted gene …
Read PDFGlobal climate changes cause severe floods that hinder plant development and breeding. The phenomenon in which a single plant can significantly alter its leaf shape structure and physiology based on surrounding environments is known as …
Read PDFChronic kidney disease (CKD) is a leading and rapidly rising contributor to global mortality, yet actionable molecular targets remain limited. Here, we integrated human kidney single-cell RNA sequencing (scRNA-seq) with genetic association and causal inference …
Read PDF<h4>Background</h4>Paraneoplastic pemphigus (PNP) is a highly fatal autoimmune blistering disease that commonly occurs in patients with underlying benign or malignant neoplasms. It poses significant challenges for diagnosis and treatment. To date, the cellular and molecular …
Read PDFProstate cancer exhibits a strong familial association, and its heritability indicates a significant contribution from germline variants. While genome-wide association studies (GWAS) have identified common germline variants associated with prostate cancer risk, translating these statistical …
Read PDFAlternative splicing (AS) plays a critical role in generating cellular transcriptomic heterogeneity. While single-cell RNA sequencing (scRNA-seq) has become a standard approach for exploring this heterogeneity, it remains challenging to accurately characterize splicing changes at …
Read PDFTissue makeup depends on the local cellular microenvironment. Spatial single-cell genomics enables scalable and unbiased interrogation of these interactions. Here we introduce Nicheformer, a transformer-based foundation model trained on both human and mouse dissociated single-cell …
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