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The 5-year overall survival rate for hepatocellular carcinoma (HCC) patients remains below 20%. Alterations in the extracellular matrix (ECM) are increasingly recognized as central drivers of HCC initiation and progression. This study applied a system …
Read PDFDiabetic foot ulcers (DFU) is severe complications of diabetes mellitus, affecting 15–25% of diabetic patients and imposing substantial healthcare burdens. Lactylation has emerged as a critical regulator in inflammation and wound healing processes. However, the …
Read PDF<h4>Background</h4>Low-density lipoprotein (LDL) is a critical regulator of lipid metabolism and has been implicated in the development and progression of various malignancies. However, its specific roles and mechanisms in the ovarian cancer tumor microenvironment (TME) …
Read PDFSkeletal stem cells (SSCs) are well characterized in humans and mice, providing valuable insights into bone development and regeneration. However, their identification in poultry, particularly in chicken embryos, remains limited. In the present study, we …
Read PDF<h4>Background</h4>Macrophages are key regulators within the lung adenocarcinoma (LUAD) tumor microenvironment, and their functional states and interaction patterns are closely linked to prognosis and immunotherapy response. However, macrophage-associated risk models derived from single-cell features remain …
Read PDFIschemic stroke is a cerebrovascular disease that can cause long-term neurological impairment, dementia, or death. It is the third most common cause of disability and the second leading cause of death worldwide. The aim of …
Read PDFMicroRNAs (miRNAs) are pivotal post‑transcriptional regulators whose single‑cell behavior has remained largely inaccessible due to technical barriers in single-cell small‑RNA profiling. We present SiCmiR, a two‑layer neural network that predicts miRNA expression profiles from only …
Read PDFRecent advancements in single-cell DNA methylation (scDNAm) sequencing technologies have enabled the profiling of epigenetic landscapes at unprecedented resolution, offering insights into cellular heterogeneity, differentiation and evolution. Trajectory inference, which orders cells along pseudotime, allows …
Read PDFBenign prostatic hyperplasia (BPH) is an age-related prostate disorder with incompletely defined mechanisms. We integrated laser capture microdissection RNA sequencing, public single-cell RNA sequencing, public spatial transcriptomics, human tissue validation, mouse-model analyses, and <i>in vitro</i> …
Read PDF<h4>Background</h4>Microsatellite stable (MSS) gastric cancer (GC) responds poorly to immunotherapy and exhibits heterogeneous outcomes. Histone lactylation plays a critical role in cancer progression. However, the prognostic and therapeutic potential of a lactylation-related gene signature (LRGS) …
Read PDFPURPOSE: Dysregulated cholesterol metabolism has emerged as a crucial driver of hepatocellular carcinoma (HCC) progression and immunotherapy resistance. This study aimed to delineate the single-cell landscape of cholesterol metabolism in HCC and identify key molecular …
Read PDFWe develop Spatial Perturb-Seq, an in vivo CRISPR technology that interrogates multiple genes within single cells of intact tissues, compatible with both sequencing-based and probe-based spatial technologies. We apply Spatial Perturb-Seq to knock out risk …
Read PDF<h4>Background</h4>Type 1 diabetes (T1DM) is a T cell-mediated autoimmune disease. The sphingosine-1-phosphate (S1P) signaling pathway regulates lymphocyte trafficking, but its status in T1DM remains poorly characterized. This study investigated S1P and sphingosine-1-phosphate receptor 1(S1PR1) alterations …
Read PDFCell-cell interactions influence gene expression in the tumor microenvironment, yet despite advances in spatial transcriptomics that enable in situ expression profiling, current computational tools remain limited in quantitatively resolving single-cell and multicellular interaction effects across …
Read PDF<b>Background</b>: Tumor invasion is the central barrier to effective immunotherapy in lung adenocarcinoma (LUAD) and glioblastoma. Cell adhesion signaling critically shapes tumor-microenvironment interactions, yet the upstream regulators coordinating these invasive programs at single-cell resolution remain …
Read PDFUnderstanding the development and differentiation of cardiac progenitor cells during the initial stages of embryogenesis is central to a complete understanding of vertebrate heart development. In zebrafish, cardiac specification begins during gastrulation; however, the single-cell …
Read PDFIncreasing prevalence of Alzheimer's disease, driven by population aging, highlights the need to investigate its underlying molecular mechanisms. Within this context, long non-coding RNAs (lncRNAs) have emerged as a key regulatory layer. To advance the …
Read PDFDendritic cells (DCs) are essential for the initiation and regulation of antitumor immune responses. Although DC vaccines hold great promise in personalized cancer immunotherapy, their efficacy remains limited by tumor-induced immunosuppression and insufficient DC maturation …
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