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Cutaneous T-cell lymphoma (CTCL) is a type of lymphoma that affects the skin and has various subtypes with different features. It can be either slow-growing or aggressive and currently has limited treatment options, causing a …
Read PDFSingle-cell multiomics (scMultiomics) technologies and methods encompassing transcriptomics, genomics, epigenomics, proteomics, and metabolomics, together with associated computational tools have profoundly revolutionized disease research, enabling unprecedented dissection of cellular heterogeneity and dynamic biological responses. The use …
Read PDF<h4>Summary</h4>Recent advances in single-cell analysis have introduced new computational challenges. Researchers often need to use multiple analysis tools written in different programming languages while managing version conflicts between related packages within a single workflow. For …
Read PDFCancer remains one of the leading causes of mortality worldwide, accounting for ≈10 million deaths annually. Critically, it is metastasis and not the primary tumour that causes most of these deaths. Understanding the mechanisms behind …
Read PDFMass spectrometry imaging enables spatially resolved, label-free detection of metabolites in tissue and culture systems, providing insight into their metabolic landscapes and spatial distribution. However, conventional approaches often lack the spatial resolution and specificity needed …
Read PDFThe single-cell sequencing revolution enables simultaneous molecular profiling of various modalities across thousands of individual cells, allowing scientists to investigate the diverse functions of complex tissues. Among all the analysis steps, assigning individual cells to …
Read PDFFetal skeletal muscle development involves coordinated interactions among myogenic, stromal, vascular, and immune compartments, yet the cellular and molecular programs guiding tissue maturation remain incompletely understood. To address this, we generated a high-resolution single-cell atlas …
Read PDFSingle-cell transcriptomics is reshaping the study of cellular physiology and pathology by revealing fine-grained cell functions and states. Interpreting these signals across cell types, organs, and species benefits from curated gene sets and tools for …
Read PDF<h4>Background</h4>Chronic inflammation is a key driver of glioma progression, but its cellular organization within the tumor microenvironment remains poorly understood.<h4>Methods</h4>This study employed an integrated multi-omics analysis strategy, combining bulk transcriptomics, proteomics, and glioma cell line …
Read PDFNumerous studies have identified a large number of miRNA editing sites via deep sRNA sequencing profiling of tissue samples. However, the single-cell landscape of miRNA editing patterns has remained largely unknown to date. To investigate …
Read PDFBACKGROUND: Lymph node metastasis (LMN) is a key factor in the poor clinical prognosis of patients with gastric adenocarcinoma (GAC), but the underlying cellular regulatory networks and molecular mechanisms are not fully understood. METHODS: A …
Read PDF<b>Background</b>: The cochlea is a specialized sensory organ essential for hearing. To elucidate its cellular and molecular architecture and prioritize candidate genes associated with hearing loss (HL), we constructed a cross-species single-cell transcriptomic atlas of …
Read PDFOver the past 2 to 3 years, mass-spectrometry-based single-cell proteomics (SCP) has experienced transformative improvements in microfluidic and robotic sample preparation, innovative MS1- and MS2-based multiplexing strategies, and specialized hardware (e.g., timsTOF Ultra 2, Astral), …
Read PDF<h4>Background</h4>Giant cell tumor of bone (GCTB) presents considerable complexity in tumor microenvironment (TME) because of its intricate intercellular heterogeneity and the presence of an immunosuppressive milieu. In order to understand the complex gene expression patterns …
Read PDFThe immunosuppressive and therapy-resistant nature of glioblastoma (GBM) is fundamentally driven by the profound spatiotemporal and metabolic heterogeneity of tumor-associated macrophages (TAMs). This review proposes a spatiotemporal-metabolic axis as an integrative framework to decipher the …
Read PDF<h4>Background</h4>Atherosclerosis (AS) is a widespread cardiovascular disorder that constitutes a major contributor to global morbidity and mortality, thereby imposing significant economic burdens on healthcare systems worldwide. Efferocytosis, the phagocytic removal of apoptotic cells, serves as …
Read PDFTumor heterogeneity is one of the central challenges in oncology, contributing to treatment resistance and disease recurrence. Bulk RNA sequencing has advanced understanding of tumor biology, yet its averaging effect conceals cell type-specific alterations. Single-cell …
Read PDFCardiac morphogenesis requires the intricate coordination of different cell types and molecular cues. Coronary vessel formation is essential for heart development, yet how coronary vessels grow and contribute to ventricular wall formation remains poorly understood. …
Read PDFCells within the same tissue exhibit heterogeneity, influenced by several factors including enhancers. Enhancers regulate precise spatial and temporal patterns of gene expression by switching between active or inactive states. Therefore, it is imperative to …
Read PDF<h4>Introduction</h4>Dysregulation of the peripheral immune system may increase Alzheimer's disease (AD) risk, but the underlying cell type-specific mechanisms remain unclear.<h4>Methods</h4>We conducted Mendelian randomization and colocalization analyses of 4489 genes using single-cell expression quantitative trait locus …
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