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Italian ryegrass (Lolium multiflorum Lam.) is an important forage grass, providing a major source of feed for ruminants in temperate regions. Due to its highly heterozygous and repeat-rich genome, high-quality chromosome-level genome assemblies are scarce …
Read PDF<i>Leishmania panamensis</i> is a causative agent of leishmaniasis in Latin America, with significant incidence in Panama and Colombia. The first annotated genome draft for this parasite remained incomplete due to its highly repetitive nature. Here, …
Read PDFHighland potato areas in East Java, Indonesia-Sumber Brantas and Tosari-face severe late blight caused by <i>Phytophthora infestans</i>. We profiled phyllosphere fungal communities (phylloplane plus leaf endosphere) on symptomatic potato leaves and examined how management contexts …
Read PDFAutism Spectrum Disorder (ASD) is a complex neurodevelopmental disorder marked by difficulties in communication, social interaction, and restricted, repetitive behaviors. The gut microbiota has emerged as a key factor in the gut-brain axis relevant to …
Read PDFNanopore direct RNA sequencing (DRS) offers distinct advantages for transcriptome analysis over the traditional high-throughput RNA sequencing methods by preserving native RNA modifications, eliminating polymerase chain reaction bias, and simplifying the workflow. However, its high …
Read PDFComplete mitochondrial genomes provide powerful insights into evolutionary relationships, phylogeography, and emerging epigenetic regulation, yet remain poorly characterized for many fishes. Here, we report the first complete mitogenome of the square-head catfish Chaca chaca and …
Read PDFNucella lapillus is an important player in rocky shore food chains and has been a focal organism of ecological and evolutionary studies for decades. Despite poor dispersal, they have a broad geographic range, which makes …
Read PDFGenomics research is essential for understanding the transmission and evolution of bacterial antimicrobial resistance. The development of nanopore long-read sequencing has revolutionized our understanding of the prevalence, spread, and dynamic evolution of bacterial ARGs. This …
Read PDFIn this work, we report the complete genome sequence of <i>Bacillus velezensis</i> strain Tcba05. This strain is a potent biocontrol agent against multiple plant diseases in Taiwan, including asparagus bean <i>Fusarium</i> wilt and cabbage black …
Read PDF<h4>Background</h4>Symptomatic apical periodontitis (SAP) is a painful inflammatory disease driven by root canal infection. A detailed understanding of its microbial ecology, compared to a noninfectious baseline, is needed.<h4>Aims</h4>This study aimed to characterize the microbial ecology …
Read PDF<h4>Motivation</h4>Oxford Nanopore Technologies' devices, such as MinION, permit affordable, real-time DNA sequencing, and come with targeted sequencing capabilities. Such capabilities create new challenges for metagenomic classifiers that must be computationally efficient yet robust enough to …
Read PDFThe genus <i>Kluyvera</i> is presumed to be the original source of <i>bla</i><sub>CTX-M</sub> genes, which encode one of the most prevalent extended-spectrum β-lactamases. We report the draft genome sequence of <i>Kluyvera ascorbata</i> strain KHSU-R5-7, a CTX-M-5 …
Read PDF<h4>Purpose</h4>To describe an unusual case of bilateral, hypertensive uveitis in which a syphilis genome with antimicrobial resistance was identified in the aqueous humor.<h4>Methods</h4>A retrospective review of patient records was performed for clinical data. An aqueous …
Read PDF<b>Background:</b> Structural variants (SVs) play a significant role in gene function and are implicated in numerous human diseases. With advances in sequencing technologies, identifying SVs through whole-genome sequencing (WGS) has become a key area of …
Read PDFRNA modifications are essential regulators of gene expression and cellular function, modulating RNA stability, splicing, translation, and localization. Dysregulation of these modifications has been linked to cancer, neurodegenerative disorders, viral infections, and other diseases. Precise …
Read PDFThe community of microorganisms occurring in the animal gut, known as the gut microbiota, is closely connected to host health. It is essential for metabolic processes, digestion, and defense against pathogens. Knowledge about the composition …
Read PDFRNA editing and maturation are critical regulatory mechanisms in plant organelles, yet their quantification remains technically challenging. Traditional Sanger sequencing lacks sensitivity and reproducibility, whereas advanced next-generation sequencing (NGS) approaches, such as rRNA-depleted long non-coding …
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