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Spoladea recurvalis is a major pest of amaranth, capable of causing up to 100% yield loss if not controlled. Current control measures rely on synthetic chemicals; however, these chemicals are unsafe due to the crop’s …
Read PDFSomatic cell nuclear transfer (SCNT) holds promise for animal cloning but remains limited by low efficiency and phenotypic abnormalities, often attributed to incomplete nuclear reprogramming. This study presents an integrative genomic and epigenomic analysis of …
Read PDFSevere acute respiratory infection (SARI) remains one of the leading causes of morbidity and mortality worldwide. Multiple viruses can cause this infection. Sequencing technologies hold great promise for detecting viral pathogens. This proof-of-concept study aimed …
Read PDFHerein, we report the nearly complete genome sequences of Actinobacteriota <i>Kitasatospora</i> sp. CMC57 and <i>Streptomyces</i> sp. CMC78, which were isolated from agricultural soils in Japan.
Read PDFAntimicrobial-resistant (AMR) <i>Klebsiella</i> spp., the common agents of nosocomial infections, are on the rise and becoming increasingly dangerous, with tigecycline-resistant strains posing a serious clinical threat. This study aimed to characterize isolated tigecycline-resistant strains from …
Read PDFThe isolation of locally adapted rhizobial strains with high symbiotic activity represents an effective strategy for increasing soybean yield under extreme environmental conditions. In this study, seven novel strains were isolated from nodules of soybeans …
Read PDF<h4>Background</h4>Whole-genome sequencing (WGS) provides critical insights into the respiratory syncytial virus (RSV) transmission and any emerging mutations that could impair the efficacy of monoclonal antibodies or vaccines that have been recently licenced for clinical use …
Read PDFNorwegian Red (NR) cattle are the main dairy breed in Norway, bred according to a broad breeding goal including health and fertility since the 1970s. Genomic studies on NR cattle have relied on the public …
Read PDFThe recently described genus <i>Candolleomyces</i> (Basidiomycota, Agaricales, Psathyrellaceae) is now recognized as a distinct taxonomic group separate from <i>Psathyrella</i>. Currently, no fully assembled and accurately annotated genomes of <i>Candolleomyces</i> species are available, limiting our understanding …
Read PDF<b>Background/Objectives</b>: Advances in nanopore sequencing have opened new avenues for studying DNA methylation at single-base resolution, yet their application in epigenetic ageing research remains underdeveloped. <b>Methods</b>: We present a novel framework that leverages the unique …
Read PDFNile tilapia (<i>Oreochromis niloticus</i>) is an indispensable source of high-quality protein worldwide. Along with the exponential expansion of tilapia aquaculture, several novel pathogenic viruses have emerged, and some cause significant economic losses. Unfortunately, there is …
Read PDF<h4>Objectives</h4>Hygroryza aristata, an aquatic plant native to Southeast Asia, shows a high degree of adaptability to aquatic environments. H. aristata, which belongs to the Oryzeae tribe and is closely related to rice (Oryza sativa), holds …
Read PDFHuman adenovirus type 3 (HAdV-3) in the species <i>Mastadenovirus blackbeardi</i> is a frequent cause of hundreds of respiratory infections in Japan, with outbreaks varying in clinical severity. Such a high frequency of cases could be …
Read PDFThe data presented in this study are metagenomic profiles of human gut microbiome deduced from frozen fecal samples using two different sequencing chemistries namely, Illumina and Oxford Nanopore Technologies (ONT). The generated data is obtained …
Read PDF<h4>Background</h4>Timely and accurate identification of bloodstream pathogens is critical for targeted antimicrobial therapy in sepsis. Conventional blood cultures remain the Standard-of-Care (SoC) for pathogen identification but are limited by low sensitivity and prolonged turnaround times, …
Read PDFLancefield group C/G streptococci (GCS/GGS) are increasingly recognized as significant human pathogens that cause a disease spectrum similar to <i>Streptococcus pyogenes</i>. Despite their high clinical burden in the UK, their genomic diversity remains poorly understood. …
Read PDF<i>Listeria monocytogenes</i> is a major foodborne pathogen, responsible for severe listeriosis outbreaks associated with contaminated foods. This study reports the comparative genomic analysis of three novel <i>L. monocytogenes</i> strains C5, A2D9 and A2D10, obtained from …
Read PDFGIY-YIG homing endonucleases are mobile genetic elements found in phage, bacterial, and organellar genomes. Their modular architecture and sequence-tolerant DNA cleavage properties likely represent evolutionary adaptations to tolerate genetic drift at target sites and enable …
Read PDFNucleotide modifications deviate nanopore sequencing readouts, therefore generating artifacts during the basecalling of sequence backbones. Here, we present a reference-guided, iterative approach to polish modification-disturbed basecalling results. We show that such an approach is uniquely …
Read PDFSoil microbiome profiling is crucial for understanding microbial diversity and its roles in ecosystem functioning and agricultural productivity. Recent advancements in high-throughput sequencing, particularly Pacific Biosciences (PacBio) and Oxford Nanopore Technologies (ONT), offer long-read sequencing …
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