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Two near-complete genomes of genotype II African swine fever viruses (ASFV) recovered from domestic pigs in Mbozi district, Tanzania, in 2017 were generated using tiled amplicon Oxford nanopore sequencing. These two ASFV genomes described in …
Read PDFThis study identified and characterised three Genomoviruses during a circular DNA-enriched sequencing project aimed at assessing the evolution of Cassava mosaic begomoviruses in Nigeria. Using a combination of rolling circle amplification, Oxford Nanopore Sequencing and …
Read PDFAmong the various genotypes of the human papillomavirus (HPV), the HPV16 genotype is the most oncogenic by far, and some (sub)lineages may be associated with an increased risk of cancer. Thus, characterizing the full genome …
Read PDF<h4>Background</h4>Sanger sequencing remains the gold standard for characterizing genetic variants in short DNA fragments (<700 bp). However, the increasing demand for short TATs and high sensitivities in variant detection, particularly in oncohematology, is driving the …
Read PDFNipah virus (NiV) is a deadly zoonotic pathogen in Southeast Asia causing severe respiratory and encephalitis symptoms with a high fatality rate. Whole-genome sequencing (WGS) is crucial for tracking transmission, conducting epidemiological analyses, and understanding …
Read PDFDuring the COVID-19 pandemic, next-generation sequencing (NGS) has been instrumental for public health laboratories in tracking severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) mutations and informing responses. Illumina systems and Oxford Nanopore Technologies (ONT) have …
Read PDFProtein-protein interactions (PPI) play a key role in host-pathogens interaction studies, as proteins are essential to many cellular mechanisms. The yeast two-hybrid (Y2H) approach is a well-established method for high-throughput PPI screening and mapping of …
Read PDF<h4>Purpose</h4>This study aimed to comprehensively analyze the global landscape, trends, and research focus of nanopore sequencing technology in the field of pathogenic microorganism diagnosis using bibliometric analysis.<h4>Methods</h4>Literature published between January 2014, and December 2024, was …
Read PDFPlasmid construction is central to life science research, and sequence verification is arguably its costliest step. Long-read sequencing has emerged as a competitor to Sanger sequencing, with the principal benefit that whole plasmids can be …
Read PDFTrypanosoma lainsoni, Trypanosoma platydactyli, and Trypanosoma scelopori are kinetoplastid flagellates belonging to the LSRM clade of the genus Trypanosoma. These parasites have a unique mitochondrial DNA structure, the kinetoplast, comprising catenated maxicircles and minicircles. However, …
Read PDFCowpea (<i>Vigna unguiculata</i>) is a versatile legume crop providing a critical source of grain, vegetable and forage globally. Cultivated cowpea is classified into two main subspecies, subsp. <i>sesquipedalis</i> for fresh-pod vegetable and subsp. <i>unguiculata</i> for …
Read PDFPhytoplasmas are non-culturable obligate intracellular bacteria that cause considerable economic losses in agriculture. Genome sequencing provides crucial insights into their biology and vector dependence. However, genome studies on phytoplasmas are often hampered by their low …
Read PDF<i>Limosilactobacillus fermentum</i> NS2301G2, isolated from fermented kimchi, underwent nanopore and Illumina sequencing, revealing a 2,077,637 base pair circular chromosome with 51.51% GC content, providing valuable genetic insights into lactic acid bacteria that contribute to food …
Read PDF<b>Background</b>: Testicular development and spermatogenesis are intricate biological processes controlled by a coordinated transcriptional network. However, comprehensive characterization of full-length transcripts and non-coding RNAs (ncRNAs) during porcine testicular sexual maturation remains limited. <b>Methods</b>: This study …
Read PDFThe detection of high-consequence viral pathogens is essential for spillover prevention and reduction in transmission but is limited by the low sensitivity of next-generation sequencing technology. Low-titer field samples from a variety of hosts are …
Read PDFDNA methylation plays a key role in gene regulation, genome stability, bacterial adaptation, and many other essential cellular processes. Thanks to nanopore sequencing technology, it is now possible to detect these modifications during sequencing without …
Read PDF<h4>Introduction</h4>Temporomandibular joint disorders (TMJD) are common, affecting approximately 30% of the population. While the aetiology of TMJD has been considerably discussed, one aspect that has not been thoroughly investigated is the potential occurrence and role …
Read PDF<b>Background/Objectives:</b> The diagnosis of prosthetic joint infection remains difficult. Microbiological cultures frequently have false-positive and false-negative results. This study investigates whether rapid nanopore sequencing can be used to aid the identification of bacteria causing prosthetic …
Read PDF<h4>Background</h4>Advances in our technological capacity to interrogate CNS tumor biology have led to the ever increasing use of genomic sequencing in diagnostic decision making. Presently, CNS tumors are classified based on their epigenetic signatures, leading …
Read PDFRibosomes are key molecular machines that translate mRNA into proteins. Mitoribosomes are specific ribosomes found in mitochondria, which have been shown to be remarkably diverse across eukaryotic lineages. In plants, they possess unique features, including …
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