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Drug discovery remains a protracted and capital-intensive process, primarily hindered by inefficiencies in drug screening. Microfluidic technology provides a promising approach for <i>in vitro</i> drug screening, enabling physiologically relevant, high-throughput, and cost-effective analysis by mimicking …
Read PDFSingle-cell RNA sequencing (scRNA-seq) in multi-condition experiments enables the systematic assessment of treatment effects. Analyzing scRNA-seq data relies on linear dimensionality reduction (DR) methods like principal component analysis (PCA). These methods decompose high-dimensional gene expression …
Read PDF<h4>Background</h4>Electrophysiological features of glioblastoma cells (GBCs) remain largely elusive, challenging our comprehension of glioblastoma pathophysiology. Spiking GABAergic-oligodendrocyte-progenitor (OPC) tumor cells were recently described in IDH-mutant glioma, correlating with prolonged patient survival. Here, we characterize single-cell …
Read PDF<h4>Background</h4>Osteosarcoma (OS) is a malignant primary bone tumor developing from primitive mesenchymal cells. Glycosylation is important in the adhesion, metastasis and transformation of cancer cells. Nevertheless, the investigation of glycosylation-related genes (GRGs) in OS has …
Read PDF<h4>Introduction</h4>Intra-tumoural heterogeneity contributes to treatment resistance and disease progression in cancer. Single-cell RNA sequencing (scRNA-seq) enables profiling of cellular diversity in the tumour microenvironment. However, current protocols for generating single-cell 3'gene expression data require intact …
Read PDFFollowing thymic egress, CD8<sup>+</sup> T cells undergo post-thymic maturation to transition from recent thymic emigrants (RTEs) to mature naive T cells. Although RTEs are phenotypically and functionally distinct from mature naive CD8<sup>+</sup> T cells, most …
Read PDFBACKGROUND: Breast cancer is one of the most prevalent malignant tumors among women, with its metastasis serving as a leading cause of mortality in affected patients. Recent studies have increasingly highlighted the significant role of …
Read PDFSingle-cell RNA sequencing (scRNA-seq) provides an instantaneous snapshot of the transcriptional state of a cell, which results from the simultaneous activity of many cellular processes. In this issue of Cell Genomics, Chen et al.<sup>1</sup> describe …
Read PDFThiols serve indispensable biochemical functions across catalysis, redox homeostasis and energy metabolism. However, profiling multiple thiols at the single-cell level remains challenging due to their trace amount and susceptibility to oxidation. Herein, we report an …
Read PDFProtein prenylation is a widespread post-translational modification (PTM) that regulates membrane association and signaling; dysregulation of this process leads to a variety of diseases. Metabolic labeling with probes containing bioorthogonal functionality has revolutionized the study …
Read PDFSystematic whole-protein screening and comprehensive profiling of antigen-specific CD4<sup>+</sup> T cells are crucial for advancing vaccine design and cancer immunotherapies, yet remain technically challenging. Here, we present a high-throughput platform that utilizes large-scale class II …
Read PDFHepatocellular carcinoma (HCC) represents one of the most prevalent malignancies worldwide and poses a critical public health challenge due to difficulties in early diagnosis, therapy resistance, and high mortality rates. The complex tumor microenvironment (TME) …
Read PDFMantle cell lymphoma (MCL) is a B-cell malignancy with a chronically relapsing clinical course and pronounced genetic heterogeneity. To investigate the clonal dynamics underlying early disease relapse, we performed single-cell RNA sequencing of paired tumor …
Read PDFSingle-cell technologies have revolutionized our ability to interrogate biological systems at unprecedented resolution, revealing complex cellular heterogeneity and dynamic processes that underlie development, disease, and immune responses. However, the high dimensionality and nonlinear structure of …
Read PDF<h4>Background</h4>Microvascular invasion (MVI) is a critical determinant of early recurrence and poor prognosis in hepatocellular carcinoma (HCC). The cellular and molecular mechanisms driving MVI, particularly the role of endothelial heterogeneity, remain incompletely understood.<h4>Methods</h4>We integrated single-cell …
Read PDFDisulfidptosis, a newly identified form of programmed cell death, has emerged as a promising therapeutic target for tumors. However, the current research in this field is hindered by the absence of precise and quantifiable biomarkers …
Read PDFUnderstanding cell state transitions and their governing regulatory mechanisms remains one of the fundamental questions in biology. We develop a computational method, state transition inference using cross-cell correlations (STICCC), for predicting reversible and irreversible cell …
Read PDFAdipose tissue plasticity and functional heterogeneity play a central role in maintaining energy homeostasis, and their malfunction leads to metabolic disorders such as obesity, diabetes, and cardiometabolic disease. Rapid, single-cell metabolic imaging of intact fat …
Read PDFImmune checkpoint blockade has shown benefit in some Triple-negative breast cancer (TNBC) patients, but responses are variable. BRCA1-mutated TNBC represents a biologically distinct subgroup, potentially differing in immunogenicity and immunotherapy responsiveness. However, immune microenvironment differences …
Read PDF<h4>Introduction</h4>The formation and homeostatic maintenance of cranial sutures rely on cellular activities within the suture mesenchyme. However, how mesenchymal stem/stromal cells (MSCs) rapidly and extensively contribute to suture and cranial development remains insufficiently explored.<h4>Methods</h4>We integrated …
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